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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
phosphoglycerate kinase 1
- 亚型:
IgG
- 形态:
liquid
- 保存条件:
负20摄氏度
- 克隆性:
Polyclonal antibody
- 标记物:
Non-conjugated
- 适应物种:
Human, Mouse
- 保质期:
6个月
- 抗原来源:
Rabbit
- 目录编号:
Q9WVH4
- 级别:
纯化级别
- 库存:
50
- 供应商:
LSM bio
- 宿主:
E. coli - derived recombinant protein
- 应用范围:
ELISA,WB,IHC
- 浓度:
≥95% as determined by SDS-PAGE
- 靶点:
phosphoglycerate kinase 1
- 抗体英文名:
anti-PGK1 antibody,PGK1 antibody
- 抗体名:
anti-PGK1 抗体,PGK1 抗体
- 规格:
100μl
PGK1抗体| PGK1 antibody
货号 PAab06354
蛋白别名 PGKA
蛋白介绍
In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). May play a role in sperm motility (PubMed:26677959).
产品描述
anti-PGK1 antibody is a Rabbit Polyclonal antibody againstPGK1..
建议稀释比例
IHC
Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using PAab06354( PGK1 Antibody) at dilution of 1:50
Western blot
HeLa cells were subjected to SDS PAGE followed by western blot with PAab06354(PGK1 antibody) at dilution of 1:1200(本抗体仅供体外科研用途,不可用于临床诊断!) <"">
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文献和实验Cell Metab:浙大吕志民团队揭示肿瘤细胞 Warburg 效应促进肿瘤免疫逃逸
,2012, PMID: 22901803; Molecular Cell, 2014, PMID: 24316223)、PGK1(Molecular Cell,2016, 2017, 2019, PMID: 26942675 , 28238651, 31492635)、KHK-A(Nature Cell Biology, 2016, PMID:2708854; Science Advances, 2019, PMID: 31032410)、PCK1(Nature, 2020, PMID
信的。 那如何来选择合适的内参呢? 首先,是内参的获得。常见内参获取的途径有如下三个: 途径一:通过查询相关文献,获取内参。 途径二:选择常用的多个内参进行实验验证。较常见内参基因见下图表: 途径三:通过查询 ICG 库,获取内参 网址:http://icg.big.ac.cn/index.php/Homo_sapiens 网站中搜集了文献报道的常见细胞/组织适用的内参。 如 ICG 库中收录的肺癌常见细胞系对应的内参基因有 ACTB、PPIA、PGK1。 其次,是内参的验证。选定的内参是否满足你的
using the AllPrep DNA/RNA 96 Kit. Real-time RT-PCR analysis of the PGK1 transcript was performed using a primer-probe set that could amplify and detect both mRNA and genomic DNA sequences. The flat amplification plot for the no RT control (-RT) indicates
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