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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Yeast
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX107614
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IP, ELISA, PLA
- 浓度:
0.048 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
PGK1
- 抗体英文名:
PGK1 antibody
- 抗体名:
PGK1 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
PGK1 antibody detects PGK1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (GTX107614) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (GTX107614) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PGK1 antibody detects PGK1 protein by western blot analysis.
A. 50 μg Rat liver lysate/extract
10% SDS-PAGE
PGK1 antibody (GTX107614) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PGK1 antibody detects PGK1 protein in 1% O2-treated samples by western blot analysis.
Upper panel: PGK1 antibody (GTX107614)
Lower panel: beta Actin antibody (GTX109639)
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (1% O2 treatment for 24hr)
10% SDS PAGE
PGK1 antibody (GTX107614) dilution: 1:1000
beta Actin antibody (GTX109639) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (GTX107614) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunofluorescence analysis of methanol-fixed HeLa, using PGK1(GTX107614) antibody at 1:50 dilution.
Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX107614 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded human hepatoma, using PGK1 antibody(GTX107614) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
PGK1 antibody detects PGK1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: PGK1 protein stained by PGK1 antibody (GTX107614) diluted at 1:20.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.
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文献和实验Somatilaka BN et al., Dev Cell 2020 (PMID:32702291)
Nan H et al., Nat Commun 2019 (PMID:30664652)
Yeh YH et al., J Exp Clin Cancer Res 2018 (PMID:29587825)
Chang SJ et al., Arch Biochem Biophys 2018 (PMID:29655550)
Silva LS et al., EMBO Rep 2017 (PMID:29066459)
Wu YH et al., PLoS One 2013 (PMID:23990911)
Lee SO et al., Mol Cancer Res 2014 (PMID:24515801)
Lin LL et al., BMC Genomics 2015 (PMID:25652794)
Drusenheimer N et al., PLoS Genet 2015 (PMID:26720614)
Chen JY et al., J Nutr Biochem 2013 (PMID:24011924)
Ogawa T et al., Physiol Rep 2023 (PMID:36802195)
Kerrie-Ann McMahon et al., Elife 2021 (PMID:34142659)
Chang YC et al., Cell Death Discov 2021 (PMID:34091600)
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