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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
neuroglobin
- 亚型:
IgG
- 形态:
liquid
- 保存条件:
负20摄氏度
- 克隆性:
Polyclonal antibody
- 标记物:
Non-conjugated
- 适应物种:
Human, Mouse ,Rat
- 保质期:
6个月
- 抗原来源:
Rabbit
- 目录编号:
Q9WVH4
- 级别:
纯化级别
- 库存:
50
- 供应商:
LSM bio
- 宿主:
E. coli - derived recombinant protein
- 应用范围:
ELISA,WB,IHC
- 浓度:
≥95% as determined by SDS-PAGE
- 靶点:
neuroglobin
- 抗体英文名:
anti-NGB antibody,NGB antibody
- 抗体名:
anti-NGB 抗体,NGB 抗体
- 规格:
100μl
NGB抗体| NGB antibody
货号 PAab05718
蛋白别名
蛋白介绍
Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.
产品描述
anti-NGB antibody is a Rabbit Polyclonal antibody againstNGB..
建议稀释比例
IHC
Immunohistochemistry of paraffin-embedded human pancreas cancer using PAab05718(NGB antibody) at dilution of 1:100
Western blot
human brain tissue were subjected to SDS PAGE followed by western blot with PAab05718(NGB antibody) at dilution of 1:500(本抗体仅供体外科研用途,不可用于临床诊断!) <"">
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文献和实验://www.uniprot.org/uniprot/P68871 见网址,只在红细胞中表达! 我把β globin理解为血红蛋白中β链珠蛋白,显然只在红细胞中表达,有携氧功能。 补充一下珠蛋白的内容: 珠蛋白是一类能够通过铁卟啉环可逆性结合氧的呼吸性蛋白质,目前在脊椎动物体内已有4种类型的珠蛋白被鉴定和正式命名:血红蛋白(haemoglobin,Hb),肌红蛋白(myoglobin,Mb),脑红蛋白(neuroglobin,Ngb)和细胞
洗脱前buffer换体系:至少2个柱体积,8ml介质一般用20ml ⑤洗脱buffer洗脱蛋白:洗脱速度很快,一般第2、3管浓度最大,注意收峰 ⑥3M NaCl再生柱子:一般会洗出一个小盐峰,再生时间不可过长,否则对柱子有伤害,5个柱体积即可 (2)凝血酶酶切融合蛋白(以NGB为例) 先后使用过sigma和鼎国的凝血酶,sigma的酶效率更高。 鼎国凝血酶买来为干粉,3000U每管,溶于1ml凝血酶酶切
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