- 询价
- SPEX CertiPrep
- 国外
- ZSC-624-C
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
保存：Freezer
- 保质期：
咨询甄准
- 英文名：
Volatile Organics Combination Mix C
- 库存：
咨询甄准
- 供应商：
上海甄准
- 规格：
1 mL
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文献和实验8) for 2 h at 37°C. Load 20 µL (~20 µg) of the trypsin-digested phosphorylated protein with 30 µL of 100 mM acetic acid onto the IMAC column. Incubate the column on a slowly rotating wheel for 30 min at 37°C. Check the column periodically for leakage
Making Combination Markers for Size Standards on Southern Blots
approximately 100 � of the 1 ng/� dilution in 10 ml 1X glycerol stop mix. NOTE: Markers should be heated to 50 degrees C for 10 minutes prior to loading to melt the lambda cohesive ends. Digesting lambda DNA (BRL) with a specific restriction
Characterization of Phosphopeptides Using a Combination of Immobilized Metal Ion
Digest the phosphoprotein (~0.1 µg/µL) with trypsin (protein:enzyme ratio of 20:1 to 100:1 [w/w]) in 50 mM NH4HCO3 buffer (pH 8) for 2 h at 37°C. Load 20 µL (~20 µg) of the trypsin-digested
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