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AJE 润色服务限时 8 折起,权威机构信任 15+ 年Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Powder:2-8℃,2 years;Insolvent(母液):-20℃,6 months;-80℃,1 year
- 保质期:
Powder:2-8℃,2 years;Insolvent(母液):-20℃,6 months;-80℃,1 year
- 英文名:
IPTG
- 库存:
999
- 供应商:
北京索莱宝科技有限公司
- CAS号:
367-93-1
- 规格:
500mg/100mg/50mg/ml*1mL in Water/1g/5g/10g
| 规格: | 500mg | 产品价格: | ¥320.00 |
|---|---|---|---|
| 规格: | 100mg | 产品价格: | ¥200.00 |
| 规格: | 50mg/ml*1mL in Water | 产品价格: | ¥160.00 |
| 规格: | 1g | 产品价格: | ¥400.00 |
| 规格: | 5g | 产品价格: | ¥640.00 |
| 规格: | 10g | 产品价格: | ¥1120.00 |
IPTG 是异乳糖模拟物,能够引起乳糖操纵子的转录过程,因此能够诱导乳糖操纵子下游基因对应蛋白的表达。
In Vitro
pull down(诱导蛋白表达,E. coli BL21 cells)
For pull down, plasmid pET-N-HSP70 was transformed into E. coli BL21 cells. The expression ofrecombinant GST-HSP70 protein was induced by isopropyl-β-D-thiogalactose-pyranoside (IPTG, Solarbio, China II0130). After the cells are destroyed by ultrasound,GST-tag protein purification kit was used to obtain purified GST-HSP70 proteins. His-G3 protein (0.5 mg) and GST-HSP70 (0.5 mg) were incubated at 4˚C for 30 min,then BeyoMag Anti-GST Magnetic Beads was added to the mixture and incubated at 4˚C overnight. The elution sample was analyzed by SDS-PAGE and Western blotting.
文献来源:Zhao Z, Zhao L, Wei XF, Jia YJ, Zhu B. Skin as outermost immune organ of vertebrates that elicits robust early immune responses after immunization with glycoprotein of spring viraemia of carp virus. PLoS Pathog. 2024 Dec 9;20(12):e1012744. doi: 10.1371/journal.ppat.1012744. PMID: 39652527; PMCID: PMC11627376.
蛋白表达与纯化,E. coli BL21 (DE3),0.3 mM IPTG,37℃,3 h
The plasmids were transformed into E. coli BL21 (DE3) for expression study. The cells were grown in LB medium with ampicillin (0.1 mg/mL) at 37℃ until OD600 was 0.6–0.8. Next, protein expression was induced with 0.3 mM isopropyl-β-d-thiogalactoside (IPTG) (Beijing Solarbio Science & Technology Co., Ltd), and the cells were grown at 37℃ for further 3 h.
文献来源:Wang B, Li H, Chen Y, Chen Z, Li P, Zhang X, Lin X. Preparation and characterization of a iRGD-modified recombinant spider silk particles for antitumor polypeptide drug delivery into cancer cells. BMC Biotechnol. 2025 Aug 27;25(1):88. doi: 10.1186/s12896-025-01023-y. PMID: 40866866; PMCID: PMC12382094.


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文献和实验状态.此时,I序列在PI启动序列操纵下表达的Lac阻遏蛋白与O序列结合,阻碍RNA聚合酶与P序列结合,抑制转录起动. 当有乳糖存在时,lac操纵子(元)即可被诱导.在这个操纵子(元)体系中,真正的诱导剂并非乳糖本身.乳糖进入细胞,经b-半乳糖苷酶催化,转变为半乳糖.后者作为一种诱导剂分子结合阻遏蛋白,使蛋白构象变化,导致阻遏蛋白与O序列解离、发生转录.异丙基硫代半乳糖苷(IPTG)是一种作用极强的诱导剂,不被细菌代谢而十分稳定,因此被实验室广泛应用. 材料: 1、诱导
载体操作中最常用的几个概念: 1. The lac Z gene may be used as a simple "reporter" gene, separated from its natural promoter. For example, we might put the lac Z gene in front of a mammalian promoter
IPTG溶液 【配制方法】 IPTG为异丙基硫代-β-D-半乳糖苷(分子量为238.3),在8ml蒸馏水中溶解2g IPTG后,用蒸馏水定容至10ml,用0.22μm滤器过滤除菌,分装成1ml小份贮存于-20℃。 (责任编辑:大汉昆仑王)
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