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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 英文名:
Mitochondrial Activity Detection Kit
- 保质期:
建议收到货后避光保存于-20°C,有效期为6个月。
- 供应商:
亚科因(Abbkine)
- 保存条件:
建议收到货后避光保存于-20°C,有效期为6个月。
- 规格:
3×10 mL
线粒体活性染色试剂盒 (Mitochondrial Activity Detection Kit)
货号:KTA4003 | 规格:3×10 mL | 价格:358元 | 货期:现货(次日发货)
核心参数
- 产品类型:细胞凋亡/周期检测专用试剂盒
- 样本兼容性:活细胞、纯化线粒体制备物
- 检测原理:基于詹纳斯绿B(Janus Green B)氧化还原特性
- 保存条件:避光保存于-20°C(有效期6个月)
- 运输条件:冰袋运输(蓝冰)
试剂盒组分
- Storage Buffer
- Stain Solution
- Assay Buffer
技术优势
- 采用低毒性碱性染料詹纳斯绿B,保障细胞活性
- 氧化态呈蓝绿色特征显色,还原态为无色背景
- 严格无菌工艺处理,即开即用
- 适用于多种活细胞及线粒体制备物的功能检测
检测原理
线粒体内细胞色素氧化酶系统使染料保持氧化状态呈现特征性蓝绿色,通过光学显微镜可清晰观察颗粒状、棒状或线状的活性线粒体结构,周围细胞质中还原态染料呈无色背景。
操作注意事项
- 实验前所有试剂需提前置于4℃解冻
- 染色过程需避光操作
- 收到产品后建议分装保存以避免反复冻融
应用领域
- 细胞能量代谢研究
- 线粒体功能状态评估
- 细胞凋亡早期检测
- 药物毒性作用机制研究
警告声明:本产品仅供研究使用,不适用于人类或临床诊断。使用前请阅读完整产品说明。
促销信息:买赠好礼(具体活动详情请咨询客服)
注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。

Mitochondrial Activity Detection Kit
Catalog Number: KTA4003 | Specification: 3×10 mL | Price: ¥358 | Availability: In Stock (Ships Next Day)
Key Parameters
- Product Type: Kit for Apoptosis/Cell Cycle Detection
- Sample Compatibility: Live cells, purified mitochondrial preparations
- Detection Principle: Based on the redox properties of Janus Green B
- Storage Conditions: Store protected from light at -20°C (Shelf life: 6 months)
- Shipping Conditions: Shipped with ice packs (blue ice)
Kit Components
- Storage Buffer
- Stain Solution
- Assay Buffer
Technical Advantages
- Utilizes low-toxicity alkaline dye Janus Green B to preserve cell viability
- Oxidized state exhibits characteristic blue-green coloration; reduced state provides colorless background
- Strict aseptic processing for ready-to-use applications
- Suitable for functional detection in various live cells and mitochondrial preparations
Detection Principle
The mitochondrial cytochrome oxidase system maintains the dye in its oxidized state, displaying a characteristic blue-green color. Active mitochondrial structures (granular, rod-shaped, or filamentous) are clearly observable under optical microscopy, surrounded by cytoplasm where the reduced dye appears as a colorless background.
Operational Precautions
- Thaw all reagents at 4°C prior to experimentation
- Perform staining procedures protected from light
- Recommended aliquoting upon receipt to avoid repeated freeze-thaw cycles
Application Fields
- Research on cellular energy metabolism
- Assessment of mitochondrial functional status
- Early detection of apoptosis
- Mechanistic studies of drug toxicity
Warning Statement: This product is for research use only. Not intended for human or clinical diagnostic use. Please read the complete manual before use.
Promotion: Free gift with purchase (Contact customer service for details)
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文献和实验Mitochondrial Activity Detection Kit
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