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- 详细信息
- 申请记录(10)
- 文献和实验
- 技术资料
- 保存条件:
于-20℃,避光保存 12 个月
- 保质期:
于-20℃,避光保存 12 个月
- 英文名:
Annexin V-AbFluor™ 488/PI Apoptosis Detection kit
- 库存:
999
- 供应商:
亚科因(Abbkine)
- 规格:
50 T/100 T
| 规格: | 50 T | 产品价格: | ¥598.0 |
|---|---|---|---|
| 规格: | 100 T | 产品价格: | ¥998.0 |
Annexin V-AbFluor™ 488/PI双染细胞凋亡检测试剂盒
产品编号:KTA0002 | 检测类型:细胞凋亡/周期分析
核心参数
- 应用范围:流式细胞术/荧光显微镜 | 细胞早期-晚期凋亡动态检测
- 检测窗口:Annexin V-AbFluor™ 488 (Ex/Em=491/517nm) & PI (Ex/Em=535/617nm)
- 规格选择:
• 50T 现货 ¥598
• 100T 现货 ¥998
技术突破点
- ► AbFluor™ 488荧光系统:FITC替代方案,pH非敏感性,光稳定性提升≥40%
- ► 双标验证体系:Annexin V特异性标记膜外PS + PI核染技术,准确区分:
• 活细胞(双阴性)
• 早期凋亡(单阳性)
• 晚期凋亡/坏死(双阳性)
试剂盒配置
- Annexin V Binding Buffer (5×浓缩液)
- Annexin V-AbFluor™ 488标记探针
- 碘化丙啶染色液(PI,DNA特异性染料)
- 凋亡诱导剂A/B(阳性对照体系)
操作与储存
- 保存条件:-20℃避光储存,有效期12个月
- 运输保障:专业级蓝冰控温运输(2-8℃全程)
- 实验兼容性:适配488nm激光流式细胞仪及常规荧光显微镜系统
重要声明:
本产品仅限研究用途,不可用于临床诊断或治疗应用。使用前请确认实验方案符合所在机构生物安全规范。
检索关键词
Annexin V双染 | Annexin V/PI凋亡检测 | AbFluor™ 488标记试剂盒 | Annexin 5检测系统
注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。

Annexin V-AbFluor™ 488/PI Double Staining Apoptosis Detection Kit
Catalog Number: KTA0002 | Assay Type: Apoptosis/Cell Cycle Analysis
Key Specifications
- Applications: Flow Cytometry/Fluorescence Microscopy | Dynamic Detection of Early-Late Stage Apoptosis
- Detection Window: Annexin V-AbFluor™ 488 (Ex/Em=491/517nm) & PI (Ex/Em=535/617nm)
- Specifications:
• 50T In Stock ¥598
• 100T In Stock ¥998
Technical Innovations
- ► AbFluor™ 488 Fluorescence System: FITC alternative, pH-insensitive, ≥40% improved photostability
- ► Dual-Labeling Verification System: Annexin V-specific PS membrane labeling + PI nuclear staining for precise differentiation of:
• Viable Cells (Double Negative)
• Early Apoptosis (Single Positive)
• Late Apoptosis/Necrosis (Double Positive)
Kit Components
- Annexin V Binding Buffer (5× Concentrate)
- Annexin V-AbFluor™ 488 Labeled Probe
- Propidium Iodide Staining Solution (PI, DNA-Specific Dye)
- Apoptosis Inducers A/B (Positive Control System)
Handling & Storage
- Storage Conditions: Store at -20°C protected from light, valid for 12 months
- Shipping Assurance: Professional blue ice temperature-controlled transport (2-8°C throughout)
- Experimental Compatibility: Compatible with 488nm laser flow cytometers and conventional fluorescence microscopy systems
Important Notice:
This product is for research use only, not intended for clinical diagnosis or therapeutic applications. Please ensure experimental protocols comply with your institution's biosafety regulations before use.
Search Keywords
Annexin V Double Staining | Annexin V/PI Apoptosis Assay | AbFluor™ 488 Labeling Kit | Annexin 5 Detection System
Note: The above information may be subject to changes due to product optimization and upgrades. For specific details, please consult ACROBiosystems.
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申请记录
文献和实验Preserving Fertility: Minimizing Ovarian Lymphatic Drainage Disruption in Gynecological Cancer Surgeries.
作者:Chen, Dan, et al. 杂志名称: IF:
Bioinformatics mining and experimental validation of prognostic biomarkers in colorectal cancer.
作者:Huang, Feng, et al. 杂志名称: IF:
Banxia xiexin decoction prevents the development of gastric cancer.
作者:Zu, Guo-**u, et al. 杂志名称:World Journal of Clinical Oncology IF:3
Mechanism of ginsenoside Rb3 against OGD/R damage based on metabonomic and PCR array analyses.
作者:Li, Fuhui, et al. 杂志名称:Biomedical Reports IF:2
CYTOTOXIC EFFECT OF Brassica oleracea L. var. italica FLORETS AND MICROGREENS ON ACUTE LEUKEMIC CANCER CELL LINE.
作者:Shah, Ami, and R. Gadhvi. 杂志名称: IF:
Folate-Mediated Targeting and Controlled Release: PLGA-Encapsulated Mesoporous Silica Nanoparticles Delivering Capecitabine to Pancreatic Tumor.
作者:Tripathi, Abhay Dev, et al. 杂志名称:ACS Applied Bio Materials IF:5
ATF4/NUPR1 axis promotes cancer cell survival and mediates immunosuppression in clear cell renal cell carcinoma.
作者:Lu, Yongliang, et al. 杂志名称:Discover Oncology IF:3
Dextran‐Based Antibacterial Hydrogel Dressings for Accelerating Infected Wound Healing by Reducing Inflammation Levels.
作者:Liang, Jianhao, et al. 杂志名称:Advanced Healthcare Materials IF:10
Inhibition of HTR2B-mediated serotonin signaling in colorectal cancer suppresses tumor growth through ERK signaling.
作者:Lee, Jeong-Yun, et al. 杂志名称:Biomedicine & Pharmacotherapy IF:7
Oral administration of Sophora Flavescens-derived exosomes-like nanovesicles carrying CX5461 ameliorates DSS-induced colitis in mice.
作者:Zhang, Manqi, et al. 杂志名称:Journal of Nanobiotechnology IF:11
Development of Daruharidra (Berberis aristata) Based Biogenic Cadmium Sulfide Nanoparticles: Their Implementation as Antibacterial and Novel Therapeutic Agents against Human Breast and Ovarian Cancer.
作者:Bhatnagar, Aditi, and Abha Mishra. 杂志名称:Current Pharmaceutical Biotechnology IF:2
CUDC-907 exhibits potent antitumor effects against ovarian cancer through multiple in vivo and in vitro mechanisms.
作者:Wang, Yuanpei, et al. 杂志名称:Cancer Chemotherapy and Pharmacology IF:3
Melatonin Alleviates Vitrification Damage in Sheep Oocytes by Enhanced Mitochondrial Function.
作者:Gao, Zihao, et al. 杂志名称: IF:
Bioinformatics analysis and experimental verification of NLRX1 as a prognostic factor for esophageal squamous cell carcinoma.
作者:Zhou, Lu, et al. 杂志名称:Oncology Letters IF:2
Aloperine Suppresses Cancer Progression by Interacting with VPS4A to Inhibit Autophagosome‐lysosome Fusion in NSCLC.
作者:Guo, Weina, et al. 杂志名称:Advanced Science IF:14
Neohesperidin Dihydrochalcone Alleviates Lipopolysaccharide‐Induced Vascular Endothelium Dysfunction by Regulating Antioxidant Capacity.
作者:Nong, Yuxin, et al. 杂志名称: Immunity, Inflammation and Disease IF:3
示例: Jurkat细胞用1μM喜树碱(Camptothecin) (左)或未加药 (右)处理4h,FITC-Annexin V/PI荧光双染细胞凋亡检测试剂盒染色后,流式细胞仪荧光检测。 Annexin V-FITC单阳性细胞为早期凋亡细胞,Annexin V-FITC和PI染色双阳性的细胞为坏死细胞或者晚期凋亡。PI单染色阳性位裸核细胞。 实测数据可能会因细胞类型、细胞凋亡情况,检测仪器等的不同而存在差异,图中数据仅供参考。 流式检测细胞凋亡的数据分析方法: 1) 选中所有颗粒,将FSC
产品名称:Annexin V-FITC & PI凋亡检测试剂盒英文名称:Annexin V-FITC & PI Apoptosis Detection Kit产品分类:细胞凋亡检测试剂盒[Apoptosis Assay Kit]产品编号:A0001c检验方法:Apoptosis包 装:20T产品说明:Annexin V-FITC & PI凋亡检测试剂盒 概述产品说明在正常细胞中,磷脂酰丝氨酸只分布在细胞膜脂质双层的内侧,细胞发生凋亡早期,膜磷脂酰丝氨酸(PS)由脂膜内侧翻向外侧。Annexin V
,Annexin染色都是阳性,而坏死细胞是阴性的。那如果我只想知道凋亡细胞的百分率,是不是只要染Annexin就可以啦? midas: 坏死细胞的细胞膜都破裂了,PS也一定可以染出来! pinkhouse: Annexin-v-FITC细胞凋亡检测试剂盒是北京宝赛生物技术有限公司的产品(北京大学医学部的技术-原北医大)。 Annexin V-PI双染试剂盒是北京宝赛生物技术有限公司的产品(北京大学医学部的技术),不是宝生物公司的。其价格为:960元/50T;500元/25T。该产品技术为国内首创,销售
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