- ￥10000 - 11500
- 三一造血stemery
- RC-002-100/RC-002-100N/RC-002-500N
- 中国
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
广州亿宁生物技术有限公司
- 生长状态：
好
- 年限：
5年
- 运输方式：
-20
- 是否是肿瘤细胞：
否
- 细胞形态：
好
- 规格：
500ml
产品简介
本产品将多人份浓缩血小板混合后裂解而成，含有很多不同的细胞的因子，包括 PDGF, VEGF，EGF 等，实践证明，血小板分泌的细胞生长因子能够对多种细胞起到支持生长的作用，可以完全替代胎牛血清（FBS）甚至于在支持一些细胞培养的效果上优于FBS。本产品是将多人份浓缩血小板混合后裂解而成，产品均一性好，经过了严格安全性有效性质量检控。人血小板裂解液能够支持包括间充质干细胞成骨分化，成脂分化，造血干细胞以及诱导性多能干细胞的生长等。
产品特点
- 每批次产品均混均经过严格的安全性检控
- 无菌，无支原体，地内毒素
- 营养丰富，可支持包括MSC在内多种细胞的快速增殖
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文献和实验MATERIALS AND METHODS
Isolation of UCMSCs and culture conditions
Human umbilical cords (UCs; n = 5) were obtained from consenting patients who delivered full-term (38–40 weeks) infants by
cesarean section. The study was approved by the Ethics
Committee of Tongji Medical College, Huazhong University of
Science, and Technology and followed the principles of the
Declaration of Helsinki. The UCs were rinsed with phosphatebuffered saline (PBS; HyClone, Logan, UT, USA) to remove blood
cells. Subsequently, the samples were cut into 1 cm segments, and
the UC veins, arteries, and amnion were removed. The UCs were
then minced into 1–3 mm3 pieces. The minced pieces were placed
in a 55 cm2 culture dish and incubated in DMEM/F-12 (HyClone)
supplemented with 10% fetal bovine serum (FBS; Gibco, Carlsbad,
CA, USA). After 2 weeks, the UC tissue was removed, and the
adherent cells were passaged with 0.25% trypsin (Gibco) for 1 min
at 37 °C. The obtained cells were centrifuged at 250 ×g for 5 min
and subcultured at a density of 4000 cells/cm2 in α-MEM (HyClone)
supplemented with 5% human platelet lysate (HPL; Stemery
Biotech, Fujian, China) and 100 U/mL penicillin/streptomycin
(Gibco). The concentration of HPL was determined by CCK-8
analysis (Supplementary Fig. S1). Cell passaging was performed
when the monolayer of adherent cells reached 70%–80%
confluence. All cells were maintained at 37 °C in a humidified
atmosphere with 5% CO2.
