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- 2025年07月14日
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文献和实验Microtubule Spindowns for Visual Analysis
onto coverslips are described here. I. Solutions & Supplies II. Spindown Protocol Back to protocols I. Solutions & Supplies BRB80 (1X): 80 mM PIPES, 1 mM MgCl2 , 1 mM EGTA, pH 6.8 with KOH (generally made as a 5X
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
for 2 days. 10. Rinse slides in PBS + 20 mM EDTA, 3x 10 minutes. 11. Mount in Gelvatol (+ 20 mM EDTA if desired). Storing slides at -80o C helped prevent background staining from increasing. Notes If cultured cells
Microtubule Spindowns for Visual Analysis
Back to protocols I. Solutions & Supplies BRB80 (1X): 80 mM PIPES, 1 mM MgCl2 , 1 mM EGTA, pH 6.8 with KOH (generally made as a 5X stock and stored at 4¡C) Fix : 1% glutaraldehyde in BRB80 (prepare from a 50% glutaraldehyde stock
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