1,3-二氟-5-甲磺酰基苯,1,3-Difluoro-5-

干货：如何获得更高纯度、更安全的 MSC 外泌体？

外泌体提取过程中，影响纯度的最大因素之一是杂蛋白，杂蛋白是令无数外泌体人为之头疼的痛点！ 杂蛋白来源于哪里呢？ 以 MSC 干细胞为例，在培养过程中的大量血清 (FBS)、人血小板裂解物 (HPL)、蛋白因子混合物的使用，这些都含大量外泌体杂质，其中一些杂质是蛋白因子，经过长期细胞培养也不会降解，增加了培养基的异源外泌体或者外泌体类似物，对于下游外泌体的制备带来诸多不可控的影响。 如何解决杂蛋白问题呢？ 从实验设计的角度来说，在前端杂蛋白越少，意味着后端去除杂蛋白的方法和步骤

Chromosomal Aberration Test in Human Lymphocytes

Human peripheral lymphocytes (HPL) are non-cycling primary cells (G0 cells). They are easily collectable by venipuncture. In the presence of suitable culture media and stimulants in vitro HPL enter the cell cycle and divide mitotically

Generation of a Pool of Human Platelet Lysate and Efficient Use in Cell Culture

for the preparation of human platelet lysate (HPL) by several freeze–thaw cycles has been developed, resulting in platelet fragmentation and the release of stored growth factors. By pooling up to 15 U of HPL derived from individual blood donors, a virtually