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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Corning® BioCoat™ Collagen 175cm² Rectangular Straight Neck Cell Culture Flask with Vented Cap, 5/Pack, 40/Case
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
40
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文献和实验2) Trim any subcutaneous fat and finely chop the biopsy into small pieces by rocking a number 24 scalpel blade against the bottom of the petri dish. 3) Place tissue slurry in a 25 cm2 tissue culture flask with primary fibroblast
nitrogen. Each T175 flasks of cells can be aliquoted into 6 vials. Each vial can then be plated onto 1x T25 flask. You may need to adjust these numbers according to plating efficiency. 3. ES Cell Maintenance To maintain a healthy
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
实验试剂 Buffer: Phosphate buffered saline with 0.1% bovine serum albumin and 2 mM EDTA, pH 7.4 (PBS w/0.1% BSA). Magnet (DynaMag™): See www.invitrogen. com/magnets for magnet recommendations. Culture medium: Advanced
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