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- 2026年09月27日
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- 文献和实验
- 技术资料
- 英文名：
Corning® BioCoat™ Collagen I 150mm TC-Treated Culture Dishes, 5/Pack, 5/Case
- 供应商：
合肥瑞辕生物科技有限公司
- 规格：
5CS
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文献和实验E.Z.N.A.® Protocol for Bacteria
flow-through. Then empty the collection tube and centrifuge the spin cartridge for 10 min at 4000-5000 x g to completely dry the HiBind™ matrix. 8. RNA Elution. Transfer column to a clean 15 ml collection tube (Not supplied) and elute RNA with 250
Lifecell® or CellGenix Vuelife™) 3-L Culture Bags (Baxter Lifecell® or CellGenix Vuelife™) Sampling site coupler with female luer (Charter Medical) Terumo TSCD® Sterile Tubing Welder 40μm - 80μm In-line Transfusion Filter (Pall
ES / MEF cell culture and electroporation of targeting construct
. You will need 4 x 10 cm dishes of irradiated MEFs for your electroporation on Day 7. You have 2 T-150's ongoing from which to make these 4 dishes. Follow the procedure for mitotically inactivating MEFs on Day 4. However, use both T-150s this time. Don't forget
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