- ￥2038
- BioLegend
- 301813
- 美国
- 2025年07月14日
- 免疫荧光，流式
- 小鼠
- Human, Cynomolgus, Rhesus Reported Reactivity African Green, Capuchin Monkey, Cow, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Dog, Pigtailed Macaque, Squirrel Monkey
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- 文献和实验
- 技术资料
- 抗体名：
PE/Cyanine7 anti-human CD14
- 抗体英文名：
PE/Cyanine7 anti-human CD14
- 应用范围：
免疫荧光，流式
- 宿主：
小鼠
- 供应商：
上海善然生物
- 库存：
现货
- 抗原来源：
小鼠
- 保质期：
2-3年左右
- 适应物种：
Human, Cynomolgus, Rhesus Reported Reactivity African Green, Capuchin Monkey, Cow, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Dog, Pigtailed Macaque, Squirrel Monkey
- 标记物：
PE/Cyanine7
- 克隆性：
单克隆
- 形态：
液体
- 规格：
25ug
PE/Cyanine7 anti-human CD14 Antibody
M5E2
Catalog# / Size 301813 / 25 tests
301814 / 100 tests
Clone
Regulatory Status RUO
Workshop III 329
Other Names LPS receptor
Isotype Mouse IgG2a, κ
Description CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also
known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages,
and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular
dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to
express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gramnegative pathogens, and in the upregulation of adhesion molecules and expression of
cytokines in monocytes and neutrophils.
Product Details
Verified Reactivity Human, Cynomolgus, Rhesus
Reported Reactivity African Green, Capuchin Monkey, Cow, Chimpanzee, Common Marmoset, Cotton-topped Tamarin,
Dog, Pigtailed Macaque, Squirrel Monkey
Antibody Type Monoclonal
Host Species Mouse
Immunogen Full-length human CD14 protein
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under
optimal conditions.
Concentration Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our
Certificate of Analysis online tool.)
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze
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Dendritic Cell Generation from Highly Purified CD14+Monocytes
lineage with several subsets, functions, and maturational stages. Although it is generally accepted that human DC derive from hematopoietic progenitor cells (2 -9 ), it is not clear at present whether DC cells and their precursors represent a separate
Calcitriol (1α, 25-dihydroxyvitamin D3 ) induces the expression of CD14 in mononuclear phagocytes. The mechanisms accounting for this have been unclear since the promoter of CD14 does not contain a canonical vitamin D response element (VDRE
、CD14-PE、CD19-PE、CD3-PE、CD66b-PE、CD56-PE-Cy7、CD11c-APC、 CD45-AF700、CD38-APC-AF750（每次测试 5μL），以及 CD16-FITC（每次测试 20 μL），并在冰上孵育 30 分钟。10. 在 4℃ 条件下，300 x G 离心细胞 5 分钟。11. 吸取上清液。12. 重悬到 500 μL 染色缓冲液中。13. 通过 CytoFLEX 获取结果。通过 CytoFLEX 进行数据采集：1. 创建新补偿值。2. 如适用
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