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- BioLegend
- 302916
- 美国
- 2025年08月18日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Alexa Fluor® 488 anti-human CD28
- 抗体英文名：
Alexa Fluor® 488 anti-human CD28
- 供应商：
上海善然生物科技有限公司
- 保存条件：
2-8℃
- 规格：
100tests
Alexa Fluor® 488 anti-human CD28 Antibody
CD28.2
Catalog# / Size 302916 / 100 tests
Clone
Regulatory Status RUO
Workshop V-CD28.05
Other Names T44, Tp44
Isotype Mouse IgG1, κ
Description CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the
immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T
lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a
highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major costimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell
death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen
presenting cells provides a costimulatory signal required for T cell activation and proliferation.
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文献和实验实验原理 Dynabeads® CD3⁄CD28 CTS™ offer a simple method for isolation, activation and expansion of human T cells. Firstly, CD3 T cells are separated and concentrated from the apheresis product by magnetic cell separation
分别利用 CD3 与 CD28 的功能学抗体，以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤： 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体（克隆号：145-2C11）稀释至 5μg/ml，稀释后的抗体加入到 24 孔板中，每孔 400μl，4°C 包被过夜。（注：板子在加入抗体之前，先用无菌 PBS 润洗 2-3 遍，避免干孔）； 1.2 小鼠脾脏淋巴细胞分离（次日） 1. 将 70μm
Vybrant® DyeCycle Green and Orange Stains
degradation. Incubate at 37?C for 30 minutes, protected from light. For cells stained with Vybrant® DyeCycle™ Green stain, analyze samples on flow cytometer using 488 nm excitation and green emission (Figure 3). For cells stained with Vybrant®
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