InvivoCrown In vivo macrophage

InVivoPro Anti-Mouse Ly6G/Ly6C (Gr-1) in vivo antibody, Clone RB6-8C5InVivoPro Anti-Mouse CSF1R (CD115) in vivo antibody, Clone AFS98Rat IgG2bRat IgG2a κRB6-8C5AFS98Ly6G/Ly6CCSF1RLy-6G/LY-6C, Gr-1Macrophage Colony Stimulating Factor Receptor, M-CSFR, CSF-R1IV0106 or Rat IgG2b Isotype Control IV0105 or Rat IgG2a Isotype Control PBS, pH 7.2, contains no stabilizers or preservativesPBS, pH 7.2, contains no stabilizers or preservativesMouseMouseRatRat200-500 μg per mouse; or 10-20 mg/kg. This range is based off the most recent publication data using the RB6-8C5 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications.400-500 μg per mouse; or 20 mg/kg. This range is based off the most recent publication data using the AFS98 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications.20088Ali, Niwa, et al. "Regulatory T cells in skin facilitate epithelial stem cell differentiation." Cell 169.6 (2017): 1119-1129. Miao, Yuxuan, et al. "Adaptive immune resistance emerges from tumor-initiating stem cells." Cell 177.5 (2019): 1172-1186. Ortiz-Muñoz, Guadalupe et al. "In Situ Tumour Arrays Reveal Early Environmental Control of Cancer Immunity.",NATURE 618.7966 (2023): 827-+.Lerner, Emily C. et al. "CD8+ T Cells Maintain Killing of MHC-I-negative Tumor Cells Through the NKG2D–NKG2DL Axis",Nature Cancer 4.9 (2023): 1258-1272. Hoeffel, Guillaume et al. "Sensory Neuron-Derived TAFA4 Promotes Macrophage Tissue Repair Functions",Nature 594.7861 (2021): 94-99. Bornes, Laura et al. "The Oestrous Cycle Stage Affects Mammary Tumour Sensitivity to Chemotherapy",Nature 637.8044 (2024): 195-204.Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. Clone RB6-8C5 antibody has been shown to inhibit the binding of the clone 1A8 antibody. Clone 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C.Macrophage colony-stimulating factor (M-CSF, also termed CSF-1) receptor is an integral membrane tyrosine kinase, expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage. It also triggers growth and development of mononuclear phagocytes and placental trophoblasts. M-CSF receptor is present in osteoclasts both at mRNA and protein levels. When M-CSF ligand specifically binds to the M-CSF receptor on osteoclasts, it induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues M-CSF and its receptor present as candidate therapeutic targets in states of inflammatory bone erosion.In vivo depletion of Gr-1+ myeloid cells, Flow cytometry, Immunohistochemistry(Paraffin and Frozen), Western blotIn vivo macrophage depletion, in vivo monocyte depletion, Flow cytometryprotein A or Gprotein A or GThis antibody is stable for at least 2 months when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C or -80°C. Avoid repeated freeze thaw cycles.This antibody is stable for at least 2 months when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C or -80°C. Avoid repeated freeze thaw cycles.2-8°C with blue ice2-8°C with blue iceLot specific, generally ≥ 5.0 mg/mlLot specific, generally ≥ 5.0 mg/ml12 months from the date of receipt if stored as recommended12 months from the date of receipt if stored as recommendedPBS Buffer, PH 7.2, with no carrier protein, or preservatives.PBS Buffer, PH 7.2, with no carrier protein, or preservatives.0.2 μM filtered0.2 μM filtered≤ 1.0 EU/mg, by the LAL method≤ 1.0 EU/mg, by the LAL method0.990.99可替换BioXCell产品货号为BE0213