产品封面图
文献支持

山羊抗兔IgG二抗 (HRP)

收藏
  • 询价
  • Sino Biological已认证
  • 北京
  • SSA004
  • 2026年02月06日
    avatar
  • 点击 QQ 联系

    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 形态

      液体

    • 保存条件

      -20℃ to -80℃

    • 保质期

      12个月

    • 目录编号

      SSA004

    • 级别

      免疫学试剂

    • 库存

      99

    • 供应商

      北京义翘神州科技股份有限公司

    • 抗体名

      山羊抗兔IgG二抗 (HRP)

    • 规格

      0

    IL12A & IL12B 兔多抗 (抗原亲和纯化)(产品说明)
    抗体种属:Human
    抗体靶点:IL12A & IL12B
    抗体应用:ELISA
    抗原货号:CT011-H08H
    抗原描述:Recombinant Human IL12A & IL12B protein (Catalog#CT011-H08H)
    抗体宿主:Rabbit
    抗体Ig类型:Rabbit IgG
    抗体纯化方法:Protein A & Antigen Affinity
    抗体制备:Produced in rabbits immunized with purified, recombinant Human IL12A & IL12B (rh IL12A & IL12B; Catalog#CT011-H08H; P29459-1 & NP_002178.2; Met1-Ser219 & Met1-Ser328). IL12A & IL12B specific IgG was purified by Human IL12A & IL12B affinity chromatography.
    抗体特异性:Human IL12A & IL12B
    抗体保存:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

    风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。

    • 作者
    • 内容
    • 询问日期
    图标文献和实验
    该产品被引用文献
    1, Ye, Q; et al. Expression of Programmed cell death protein 1 (PD-1) and Indoleamine
    2,3-dioxygenase (IDO) in the tumor microenvironment and in tumor-draining lymph nodes of breast cancer. Human pathology, PMID: 29447919
    2, Liao, W; et al. JIB‑04 induces cell apoptosis via activation of the p53/Bcl‑2/caspase pathway in MHCC97H and HepG2 cells. Oncology reports, PMID: 30272369
    3, Song, Z; et al. JS‑K induces G2/M phase cell cycle arrest and apoptosis in A549 and H460 cells via the p53/p21WAF1/CIP1 and p27KIP1 pathways. Oncology reports, PMID: 31002373
    4, Xian, J; et al. Progress in the research of p53 tumour suppressor activity controlled by Numb in triple-negative breast cancer. Journal of cellular and molecular medicine, PMID: 32501652
    5, Chang, J; et al. Cholecystokinin type 2 receptor in colorectal cancer: diagnostic and therapeutic target. Journal of cancer research and clinical oncology, PMID: 32488497
    6, Liu, X; et al. BRG1 protects the heart from acute myocardial infarction by reducing oxidative damage through the activation of the NRF2/HO1 signaling pathway. Free radical biology & medicine, PMID: 32950688
    7, Yuan, Y; et al. A bivalent nanoparticle vaccine exhibits potent cross-protection against the variants of SARS-CoV-2. Cell reports, PMID: 34990583
    8, Zhang, Y; et al. A second functional furin site in the SARS-CoV-2 spike protein. Emerging microbes & infections, PMID: 34856891
    9, Li, G; et al. miR-155 inhibits chondrocyte pyroptosis in knee osteoarthritis by targeting SMAD2 and inhibiting the NLRP3/Caspase-1 pathway. Journal of orthopaedic surgery and research, PMID: 35090521
    10, Huang, X; et al. Substitution of SERCA2 Cys674 aggravates cardiac fibrosis by promoting the transformation of cardiac fibroblasts to cardiac myofibroblasts. Biochemical pharmacology, PMID: 35809651
    11, Liu, H; et al. Different exposure modes of PM2.5 induces bronchial asthma and fibrosis in male rats through macrophage activation and immune imbalance induced by TIPE2 methylation. Ecotoxicology and environmental safety, PMID: 36274320 1
    2, Maso, L; et al. Nanobodies targeting LexA autocleavage disclose a novel suppression strategy of SOS-response pathway. Structure (London, England : 1993), PMID: 36240773 1
    3, Xie, Y; et al. Magnesium Ascorbyl Phosphate Promotes Bone Formation Via CaMKII Signaling. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, PMID: 37129025 1
    4, Liao, W; et al. The mechanisms of Huangqi Guizhi Wuwu decoction in treating ischaemic stroke based on network pharmacology and experiment verification. Pharmaceutical biology, PMID: 37410583 1
    5, Liu, C; et al. Isolation and enrichment of extracellular vesicles with double-positive membrane protein for subsequent biological studies. Advanced healthcare materials, PMID: 37942845 1
    6, Liao, W; et al. Investigation of the underlying mechanism of Buyang Huanwu decoction in ischemic stroke by integrating systems pharmacology-proteomics and in vivo experiments. Fitoterapia, PMID: 38580032 1
    7, Li, J; et al. Molecular Stratification and Treatment Monitoring of Lung Cancer Using a Small Extracellular Vesicle-Activated Nanocavity Architecture. Analytical chemistry, PMID: 38690989 1
    8, Yuan, Y; et al. A Streptococcus pneumoniae endolysin mutant protein ΔA146Ply elicits rapid broad-spectrum mucosal protection in mice via upregulation of GPX4 through TLR4/IRG1/NRF2 to alleviate macrophage ferroptosis. Free radical biology & medicine, PMID: 38945457 1
    9, Yu, F; et al. The histamine receptor H1 acts as an alternative receptor for SARS-CoV-2. mBio, PMID: 38953634
    20, Lin, H; et al. Simultaneous detection of membrane protein and mRNA at single extracellular vesicle level by droplet microfluidics for cancer diagnosis. Journal of advanced research, PMID: 39197817
    21, Yang, J; et al. Citreoviridin induces apoptosis through oxidative damage and inflammatory response in PC-12 cells. Toxicology and industrial health, PMID: 39437026 2
    2, Liu, J; et al. Desmoglein-2 was a novel cancer-associated fibroblasts-related biomarker for oral squamous cell carcinoma. BMC oral health, PMID: 39833796 2
    3, Xu, L; et al. ONECUT3 activates the TRIM46-NF-κB pathway to promote the development of pancreatic cancer. Biochemical and biophysical research communications, PMID: 40154001 2
    4, Park, S; et al. Tuning the Aggregation of Plasmonic Probes to Shed Light on Diagnostic Strategies. ACS sensors, PMID: 40504768 2
    5, Li, J; et al. Dietary choline deficiency potentiates Helicobacter pylori-driven gut-liver dysfunction via microbial metabolic rewiring. International immunopharmacology, PMID: 40505229 2
    6, Wang, D; et al. Aerobic exercise activates let-7e-5p through TP73-AS1 to inhibit the HMGB1/RAGE axis and alleviate asthma airway inflammation and remodeling. Cellular immunology, PMID: 40499319 2
    7, Xie, Y; et al. AGAPIR: A Novel PIWI-Interacting RNA Enhancing Post-Decompression Angiogenesis in Degenerative Cervical Myelopathy. Advanced science (Weinheim, Baden-Wurttemberg, Germany), PMID: 40820968 2
    8, Li, J; et al. Immunogenicity of differentially glycosylated Marburg virus glycoproteins expressed in mammalian and insect cells. Virology journal, PMID: 40790492
    相关实验
    • HRP 底物分类

      底物范围较广,包括二氨基联苯胺 (DAB)、氯萘酚和氨乙基咔唑等。 二氨基联苯胺/金属盐 DAB 是辣根过氧化物酶最敏感、常用的底物。它产生强烈的棕色产物,在水和醇中不溶解。多数情况下,推荐在 DAB 中加入不同的金属离子。当加入金属盐如钴、镍至底物液中,辣根过氧化物酶可以更加敏感。此类反应产物呈暗蓝灰色至黑色,且在水及醇中稳定。DAB/金属盐染色应用的组织染色范围较广。 1. 需要的溶液和特殊设备 DAB,0.05 mol/L Tris 缓冲液 (pH7.6), 0.3% (W

    • HRP标记

      问:有没有做辣根过氧化物标记的朋友啊,能不能把你们的步骤发给我。本人开始做,发现很多人用的浓度都不一样。答:1.1 试剂准备1) 10mg/mL HRP2) 0.06mol/L NaIO43) 0.16mol/L乙二醇4) 碳酸盐缓冲液(Na2CO3 0.7952g NaHCO3 1.4702g 溶于500mL蒸馏水中,调pH值到9.5)5) 2.5mg/mL NaBH46) 0.01mol/L磷酸盐缓冲液,pH7.41.2 操作步骤1. 10mg/mL取HRP VmL,溶液为棕黄色,加入

    • HRP显色

      2)将混合物覆盖在膜表面,1-2分钟,摇晃使均匀。 3)用保鲜膜把膜包起来,放入夹板中。 4)在暗室中将X光片,覆盖在膜的上面,夹好夹子,曝光1min。 5)显影、定影。 6)根据结果调整曝光时间和曝光区域,得到最佳结果。 注意:荧光在一段时间后会越来越弱。 2.DAB显色 DAB(3,3二氨基联苯胺)和HRP反应产生棕色的不溶终产物。这种棕色沉淀不溶于酒精和其它有机溶剂,对于必需使用传统复染和封固介质的免疫组化染色应用特别理想。 对于AP标记的二抗我们选用BCIP

    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    询价
    北京义翘神州科技股份有限公司(Sino Biological Inc.)
    2026年02月06日询价
    ¥500
    滨州红蓝生物科技有限公司
    2026年05月05日询价
    询价
    武汉佰瑞得生物技术有限公司
    2025年07月12日询价
    ¥100
    上海信帆生物科技有限公司
    2025年07月15日询价
    ¥300
    杭州斯达特生物科技有限公司
    2026年04月20日询价
    文献支持
    山羊抗兔IgG二抗 (HRP)
    询价