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Goat Anti-Mouse IgG H&L, HRP c

onjugated(bs-0296G-HRP)-100ul/1ml
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  • ¥180 - 1200
  • Bioss已认证
  • bs-0296G-HRP
  • 2025年10月10日
  • 产品信息以Bioss网站为准
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    • 规格

      100ul/1ml

    规格:100ul产品价格:¥180.0
    规格:1ml产品价格:¥1200.0
    产品编号bs-0296G-HRP
    英文名称Goat Anti-Mouse IgG H&L, HRP conjugated
    中文名称辣根过氧化物酶标记的羊抗小鼠IgG H&L
    英文别名Goat Anti-Mouse IgG H&L (HRP); Immunoglobulin G;
    产品应用WB=1:2000-20000, IHC-P=1:200-1000, IHC-F=1:200-1000, ELISA=1:2000-20000

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Mouse
    抗体来源Goat
    免疫原Native Mouse IgG
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein G, nonspecific adsorbed
    克隆类型Polyclonal
    浓度2.0 mg/ml
    储存液10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.
    保存条件Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

     

    应用推荐稀释比例
    {WB}{1:2000-20000}
    {IHC-P}{1:200-1000}
    {IHC-F}{1:200-1000}
    {ELISA}{1:2000-20000}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:100) instructionsand DAB staining.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:100) instructionsand DAB staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:300) instructionsand DAB staining.
    产品细节图片4
    Immobilized mouse IgG (bs-0296P, nothing else) at 0.2μg/well can bind Goat Anti-Mouse IgG H&L/HRP antibody (Cat. No. bs-0296G-HRP).
    产品细节图片5
    P‌‌araformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:300) instructionsand DAB staining.
    产品细节图片6
    P‌‌araformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:100) instructionsand DAB staining.
    产品细节图片7
    P‌‌araformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:300) instructionsand DAB staining.
    产品细节图片8
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA(Nuclear Loading Control) ) Monoclonal Antibody, Unconjugated (bsm-33035M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:200) instructionsand DAB staining.
    产品细节图片9
    P‌‌araformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:200) instructionsand DAB staining.
    产品细节图片10
    P‌‌araformaldehyde-fixed, paraffin embedded (human uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:200) instructionsand DAB staining.
    产品细节图片11
    Sample: Lane 1,3,5 : Rat Cerebrum tissue lysates Lane 2,4,6 : Human MCF-7 cell lysates Primary: Anti- GAPDH (bsm-33033M) at 1/5000 dilution Secondary: Goat Anti-Mouse IgG H&L-HRP (bs-0296G-HRP) at 1/20000~1/100000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa

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    该产品被引用文献

    [IF={{ preprint }}] {Rubina Dad. et al.Cytosolic Carboxypeptidase 5 maintains mammalian ependymal multicilia to ensure proper homeostasis and functions of the brain.Research Square.} {Western blot} {Mouse}

    [IF={{ 9.6 }}] {Anran Li. et al. Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Oxidative Damage via the miR-191-5p/DAPK1/AKT Axis in Type 2 Diabetes. Biomaterials Research. 2025 Jul;29} {WB} {Human,Rat}

    [IF={{ 9.587 }}] {Xia, Pengcheng. et al. MicroRNA-22-3p ameliorates Alzheimer’s disease by targeting SOX9 through the NF-κB signaling pathway in the hippocampus. J NEUROINFLAMM. 2022 Dec;19(1):1-19} {IHC} {Mouse}

    [IF={{ 9.5 }}] {Haoxiang Chen. et al. Living Magnetotactic Microrobots Based on Bacteria with a Surface-Displayed CRISPR/Cas12a System for Penaeus Viruses Detection. ACS APPL MATER INTER. 2023;XXXX(XXX):XXX-XXX} {WB} {Escherichia coli}

    [IF={{ 9.221 }}] {Rui Chen. et al. Tyramine signal amplification on polystyrene microspheres for highly sensitive quantification of Aflatoxin B1 in peanut samples. SENSOR ACTUAT B-CHEM. 2022 Dec;:133120} {Other} {Peanut}

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    Goat Anti-Mouse IgG H&L, HRP conjugated(bs-0296G-HRP)-100ul/1ml
    ¥180 - 1200