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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
VHH-mFC
- 形态:
Liquid
- 保存条件:
-20°C
- 克隆性:
单克隆
- 适应物种:
Human papillomavirus type 16
- 保质期:
12 months
- 级别:
一抗
- 供应商:
武汉益普生物科技有限公司
- 宿主:
Mouse
- 应用范围:
ELISA, Neutralization, WB
- 浓度:
1.97 mg/ml
- 抗体英文名:
Anti-HPV16 E7/Protein E7 Antibody (Nb2)
- 规格:
50μg/100μg/1mg
| 规格: | 50μg | 产品价格: | ¥1338.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥2288.0 |
| 规格: | 1mg | 产品价格: | ¥11148.0 |

| Product name | Anti-HPV16 E7/Protein E7 Antibody (Nb2) |
| Catalog No. | VK556013 |
| Target | E7, Protein E7 |
| Clonality | Monoclonal |
| Host species | Mouse |
| Species reactivity | Human papillomavirus type 16 |
| Applications | ELISA, Neutralization, WB |
| Isotype | VHH-mFC |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Concentration | 1.97 mg/ml |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |

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文献和实验HPV 并不是宫颈癌唯一的「元凶」?这种微生物化身「帮凶」,共同促进癌症发生
,同时进行了蛋白质的表达。 为了系统评估 hpv16 E6E7 整合和随后与沙眼梭菌合并感染对宿主调节的影响,研究人员从健康志愿者上分离出宫颈外干细胞,将其嵌入基质胶中培养 3D 类器官,并检测了来自各种 HPV 类型的 E6E7 基因。来自三个供体的子宫颈外器官检测 HPV 的 E6E7 癌基因均为阴性。这些来自健康供体的 HPV E6E7 阴性的类器官被用于研究 HPV 整合对健康宫颈组织的影响。 图片来源:Nature Communications 随后,研究人员用携带 HPV16 E6E7
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
Protocol for anti-HA antibody Western Blotting
it around for 5-10 seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes
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