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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
polypeptide, sealed storage, away from moisture
- 库存:
货期:1-2天
- 供应商:
MedChemExpress LLC
- 规格:
5 mg/10 mg
| 规格: | 5 mg | 产品价格: | ¥500.0 |
|---|---|---|---|
| 规格: | 10 mg | 产品价格: | ¥800.0 |
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HPV16 E7 (86-93) (TFA)
MCE 国际站:HPV16 E7 (86-93) (TFA)
产品活性:HPV16 E7 (86-93) TFA 是人类白细胞抗原 (HLA)-A2.1 限制的 HPV16 E7 衍生肽。HPV16 E7 (86-93) TFA 在宫颈癌中具有免疫原性。
研究领域:Others
作用靶点:Others
In Vitro: HPV16 E7 (86-93) specific T cells are expandable upon IVS (in vitro stimulation) with cognate peptide-pulsed dendritic cells (DCs) and are reactive against peptide-pulsed targets or, in case of the E711-20 epitope-specific T cells, against HPV16 E7 expressing CaSki cell line.
For peptide HPV16 E7 (86-93), the only response against K562 cells pulsed with the corresponding peptide was significantly blocked by anti-HLA class I Ab w6/32, which was not the case for HPV16 E7 expressing tumor cell line CaSki. Precursor T cells specific for HPV16 E7 (86-93) peptide is able to differentiate, at least in vitro, into HPVspecific effector cells.
HPV16 E7 peptide-loaded autologous dendritic cells (DCs) are able to stimulate a specific cytotoxic CD8+ T-cell response.
In Vivo: HPV16 E7 (86-93) peptide in IVS culture has specific T cell expansion. The HPV16 E7 (86-93) specific T cells produced does not recognize naturally HPV16 E7 expressing cell line CaSki. HPV16 E7 (86-93) peptides also has similar observations in transgenic mice. The HPV16 E7 (86-93) peptide is able to induce cytotoxic T lymphocyte (CTL) responses if loaded on the antigen presenting HLA class I molecules, but that the peptide appears not to be processed or presented by HPV16 infected cells.
相关产品:Bioactive Compound Library Plus | PEG300 | SBE-β-CD | Corn oil | Tween 80 | Polyethylene glycol 12-hydroxystearate | JC-1 | DTT | CFSE | Digitonin | Lipopolysaccharides | Thiazolyl Blue | D-Luciferin potassium salt | (2-Hydroxypropyl)-β-cyclodextrin | Methyl-β-cyclodextrin | Biotin | Monocrotaline | Auxinole | Nile Red | 3X FLAG peptide TFA | FLAG peptide | Tocofersolan | DAPI dihydrochloride | Propidium Iodide | Cremophor EL | Yoda 1 | IPTG | Calcein-AM | GsMTx4 TFA
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文献和实验HPV 并不是宫颈癌唯一的「元凶」?这种微生物化身「帮凶」,共同促进癌症发生
,同时进行了蛋白质的表达。 为了系统评估 hpv16 E6E7 整合和随后与沙眼梭菌合并感染对宿主调节的影响,研究人员从健康志愿者上分离出宫颈外干细胞,将其嵌入基质胶中培养 3D 类器官,并检测了来自各种 HPV 类型的 E6E7 基因。来自三个供体的子宫颈外器官检测 HPV 的 E6E7 癌基因均为阴性。这些来自健康供体的 HPV E6E7 阴性的类器官被用于研究 HPV 整合对健康宫颈组织的影响。 图片来源:Nature Communications 随后,研究人员用携带 HPV16 E6E7
Biolistic DNA Vaccination Against Cervical Cancer
The development of cervical cancer is associated with infection by oncogenic human papillomaviruses (HPVs), of which type 16 (HPV16) is the most prevalent in HPV-induced malignant diseases. The viral oncoproteins E6 and E7 are convenient
of the E6 and E7 oncoproteins of HPV16 is described in detail, and primers and probes that can be used to amplify these oncogenes are described. Techniques to quantify these oncogenes in infected human tissue specimens are presented, and analysis of data
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