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- 技术资料
- 抗体名:
APC anti-mouse CD144 (VE-cadherin)
- 抗体英文名:
APC anti-mouse CD144 (VE-cadherin)
- 浓度:
0.2 mg/ml
- 应用范围:
免疫荧光,流式
- 宿主:
大鼠
- 供应商:
上海善然生物
- 库存:
现货
- 抗原来源:
大鼠
- 保质期:
2-3年左右
- 适应物种:
小鼠
- 标记物:
APC
- 克隆性:
单克隆
- 保存条件:
2-8度
- 形态:
液体
- 规格:
25ug
APC anti-mouse CD144 (VE-cadherin) Antibody
BV13
Catalog# / Size 138011 / 25 µg
138012 / 100 µg
Clone
Regulatory Status RUO
Other Names Vascular endothelial-cadherin
Isotype Rat IgG1, κ
Description CD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of
the type II Cadherin family. It is an endothelial specific hemophilic adhesion molecule involved
in endothelial cell survival, migration, contact-dependent growth inhibition, and homophilic
adhesion. VE-cadherin is essential for maintaining the integrity of the endothelial barrier in
vivo.
Product Details
Verified Reactivity Mouse
Antibody Type Monoclonal
Host Species Rat
Immunogen VE-cadherin-Ig fusion protein
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography and conjugated with APC under optimal
conditions.
Concentration 0.2 mg/ml
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
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Simultaneous Immunofluorescence Detection of Cadherin and -Catenin in Mouse Tissue Sections
Both Cell-Cell adhesion and secreted signaling molecules are involved in the regulation of normal tissue development and maintenance. β-catenin seems to link cadherin-mediated Cell-Cell adhesion and Wnt signaling. Thus, its activity
分子以其独特的方式相互作用,其配体是与自身相同Cadherin分子(图2-7)。以这种方式相互作用的粘附分子除Cadherin家族的粘附分子外,还有属于免疫球蛋白超家族的CD31(PECAM)和CD56(NCAM)。 图2-7 Cadherin分子相互作用的模式图
分别利用 CD3 与 CD28 的功能学抗体,以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤: 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体(克隆号:145-2C11)稀释至 5μg/ml,稀释后的抗体加入到 24 孔板中,每孔 400μl,4°C 包被过夜。(注:板子在加入抗体之前,先用无菌 PBS 润洗 2-3 遍,避免干孔); 1.2 小鼠脾脏淋巴细胞分离(次日) 1. 将 70μm
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