- ￥5102
- BioLegend
- 123128
- 美国
- 2025年07月16日
- 免疫荧光，流式
- 大鼠
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
PerCP/Cyanine5.5 anti-mouse F4/80
- 抗体英文名：
PerCP/Cyanine5.5 anti-mouse F4/80
- 浓度：
0.2 mg/ml
- 应用范围：
免疫荧光，流式
- 宿主：
大鼠
- 供应商：
上海善然生物
- 库存：
现货
- 抗原来源：
大鼠
- 保质期：
2-3年左右
- 适应物种：
小鼠
- 标记物：
PerCP/Cyanine5.5
- 克隆性：
单克隆
- 保存条件：
2-8度
- 形态：
液体
- 规格：
100 μg
PerCP/Cyanine5.5 anti-mouse F4/80 Antibody
BM8
Catalog# / Size 123127 / 25 µg
123128 / 100 µg
Clone
Regulatory Status RUO
Other Names EMR1, Ly71
Isotype Rat IgG2a, κ
Description F4/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor
(EGF)-transmembrane 7 (TM7) family. F4/80 has been widely used as a murine macrophage
marker. It is expressed on a majority of tissue macrophages, including macrophages in the
lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans
cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the
T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4/80 has not
been identified, but it has been reported that F4/80 is required for the induction of CD8 T
cells-mediated peripheral tolerance.
+
Product Details
Verified Reactivity Mouse
Antibody Type Monoclonal
Host Species Rat
Immunogen Murine macrophages
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5
under optimal conditions.
Concentration 0.2 mg/ml
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
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Purification Of Anti Peptide Antibody
a parastaltic pump and recirculate the serum ON or just batch bind the serum ON. Wash with 5 col vols TBS. Wash with 10 col vols 0.5 M NaCl, 20mM TrisCl pH 7.4, 0.2 Triton- X-100. Wash 5 col vols TBS Elute with 0.15 M NaCl, 0.2 M Glycine
from their mothers ii. discard un-needed non-transgenic pups iii. house males and females separately f) when mice are 6 weeks old they may be mated (see 1. above) ES Cell-Mouse Chimera Breeding Based on our experience, we propose the following
Immunohistochemistry using Anti-Ganglioside Antibodies
supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab
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