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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
FITC anti-mouse CD4
- 抗体英文名:
FITC anti-mouse CD4
- 浓度:
0.2 mg/ml
- 应用范围:
免疫荧光,流式
- 宿主:
大鼠
- 供应商:
上海善然生物
- 库存:
现货
- 抗原来源:
大鼠
- 保质期:
2-3年左右
- 适应物种:
小鼠
- 标记物:
FITC
- 克隆性:
单克隆
- 保存条件:
2-8度
- 形态:
液体
- 规格:
50ug
FITC anti-mouse CD4 Antibody
RM4-4
Catalog# / Size 116003 / 50 µg
116004 / 500 µg
Clone
Regulatory Status RUO
Other Names L3T4, T4
Isotype Rat IgG2b, κ
Description CD4 is a 55 kD protein, also known as L3T4 or T4. It is a member of the Ig superfamily,
primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages
and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic
differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.
Product Details
Isotype Control FITC Rat IgG2b, κ Isotype Ctrl
Verified Reactivity Mouse
Antibody Type Monoclonal
Host Species Rat
Immunogen BALB/c mouse thymocytes
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography, and conjugated with FITC under optimal
conditions.
Concentration 0.5 mg/ml
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
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Th17 Polarization of Mouse CD4 Cells
in complete medium and use your favorite method to isolate CD4 cells. Check out Biocompare.com to find useful kits. 2. Th17 Polarization of CD4 cells 1) Coat 60×15 mm of plastic petri dishes with anti-mouse CD3ε, clone 145-2C11(2&mu
Purification of Immature CD4+CD8+ Thymocytes by Panning with Anti-CD8 Antibody
Most T lymphocytes of the immune system differentiate within the thymus along the CD4/CD8 developmental pathway by a highly ordered process termed thymic selection ( 1 , 2 ). The maturation status of thymocytes is commonly assessed
流式课堂 | 易被忽略的 CD4、CD8 共表达细胞——胸腺淋巴细胞
做过流式实验的同学,对 CD4、CD8 这两个指标都不会陌生。检测 CD4+ T 细胞和 CD8+ T 细胞分型,是流式中最常见的实验之一。 通常,我们会先检测 CD3,来圈总T淋巴细胞,再分别用 CD4 抗体和 CD8 抗体,来区分 CD4+ T 淋巴细胞(辅助或调节性T细胞)和 CD8+ T 淋巴细胞(细胞毒性 T 细胞)。 新生小鼠脾脏细胞 上图为新生小鼠脾脏细胞进行的 CD3-PE、CD4-FITC、CD8-APC 三色流式实验结果。从图中可以看出,在新生小鼠脾脏中,绝大
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