- ￥20064
- BioLegend
- 101340
- 美国
- 2025年07月14日
- 免疫荧光，流式
- 大鼠
- 小鼠
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- 技术资料
- 抗体名：
Ultra-LEAF™ Purified anti-mouse CD16/32
- 抗体英文名：
Ultra-LEAF™ Purified anti-mouse CD16/32
- 浓度：
2 mg/mL and 3 mg/mL.
- 应用范围：
免疫荧光，流式
- 宿主：
大鼠
- 供应商：
上海善然生物
- 库存：
现货
- 抗原来源：
大鼠
- 保质期：
2-3年左右
- 适应物种：
小鼠
- 标记物：
Ultra-LEAF™ Purified
- 克隆性：
单克隆
- 保存条件：
2-8度
- 规格：
25mg
Ultra-LEAF™ Purified anti-mouse CD16/32 Antibody
93
Catalog# / Size 101329 / 100 µg
101330 / 1 mg
101339 / 5 mg
101340 / 25 mg
101341 / 50 mg
101342 / 100 mg
Clone
Regulatory Status RUO
Other Names Fcγ R III/II, Ly-17
Isotype Rat IgG2a, λ
Description CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are
expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and
dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate
adaptive immune responses.
Product Details
Verified Reactivity Mouse
Antibody Type Monoclonal
Host Species Rat
Immunogen Sorted pre-B cells
Formulation 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3
mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and
expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™
solution contains no preservative; handle under aseptic conditions.
Application FC - Quality tested
IP, Block - Reported in the literature, not verified in house
Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow
cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per
million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be
titrated for optimal performance for each application.
Application Notes Clone 93 can be used for blocking of CD16/CD32 interactions with the Fc domain of
immunoglobulins, but is not the same clone as 2.4G2.
The 93 mAb is specific to the common epitope of CD16/CD32. Additional reported applications (for
the relevant formats) include: immunoprecipitation and blocking of Fc-mediated reactions in
functional studies . It is useful for blocking non-specific binding of immunoglobulin to Fc
receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with
purified anti-CD16/CD32 antibody (=1.0 µg per 10 cells in 100 µL volume) for 5-10 minutes on ice
prior to immunostaining. For highly sensitive assays, we recommend Ultra-LEAF™ purified
antibody (Cat. No. 101330) (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered)
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