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DNA连接酶在体内可以催化双链DNA中单链DNA断裂处生成磷酸二酯键,T4 DNA连接酶属于DNA连接酶的一种。本试剂盒内含有Fast Ligase以及优化后的Fast Ligation Reaction Buffer (2X)可用于快速DNA连接反应的简易系统。在常温(25℃)下反应5-10min内即可高效地完成粘性末端或平末端 DNA 插入片段与质粒载体连接反应,快速连接的连接效率相当于用T4 DNA Ligase进行常规连接1小时。连接后的产物可直接用于后续的转化实验,无需纯化。
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文献和实验Mag-Binds Plant DNA Kit Magnetic Fast Protocol
实验试剂 Regents to be provided by user 1. Absolute (96%-100%) ethanol 实验设备 Equipments to be provided by user 1. Centrifuge capable of 4,000 x g. 2. 96 well Plate 3. Water bath
immediate stabilization at room temperature. For tissues, the TissueRuptor and TissueLyser II provide fast disruption at low- to high-throughputs. The AllPrep DNA/RNA 96 Kit is intended for molecular biology applications. This product is neither intended
to up? or downregulate expression of endogenous genes in human cells and plants. This unit describes a detailed protocol for the recently described fast ligation?based automatable solid?phase high?throughput (FLASH) assembly method. FLASH enables automated high
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