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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
详见说明书
- 供应商:
广州华韵生物科技有限公司,www.huayunbio.cn
- 规格:
50次
从PCR反应液中回收DNA
产品简介:
HiPure PCR Pure Kits为PCR产物,酶促反应的DNA片段回收纯化提供一条快速的解决方案。试剂盒采用硅胶柱纯化技术,适合于从PCR产物、限制性内切酶切体系、或其它酶促反应液中回收50bp-20Kbp DNA片段。纯化的DNA可直接用于自动测序,连接,酶切,PCR,标记等。
| 产品特性与优点: 高回收效率 - DNA回收效率高达90% 低洗脱体积 - 洗脱体积可低至10ul 快速 - 过柱操作,只需10分钟 |
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文献和实验Isolation of micro-RNA (miRNA)
the frozen tissue sample in solution D using a Dounce, micro-Dounce, or a mechanical microhomogenizer. 5. Transfer the homogenate to a microcentrifuge tube. Note Hereafter, the volume of homogenate used is defined as "volume D" (e.g., in the sample
PCR amplification of variable regions (SOE-Protocol)
by special gel filtration, using S-400 sephacryl columns from PHARMACIA (Cat. No. 27-5140-01). These columns take out primers and other PCR material. It is much faster than gel purification. Besides, gel purification can give bad ligation efficiency. Combine
PCR product purification-Molecular Biology
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