- ￥3705
- 华韵生物(Huayunbio)
- HFC3600-100Tests
- 2025年07月11日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
100Tests
请联系华韵客服获取说明书。
产品简介：
| Background |
IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production.
|
| Alternate Names |
CSIF;Cytokine synthesis inhibitory factor;IL-10;Interleukin-10
|
| Swissprot |
P22301
|
| Gene ID |
3586
|
| Clone No |
JES3-9D7
|
| Host |
Rat
|
| Reactivity |
Human
|
| Application |
ICFCM
|
| Isotype |
Rat IgG1, κ
|
| Isotype Control |
PE Rat IgG1, κ Isotype Control[HRPN]
|
| Conjugation |
PE
|
| Conjugation Information |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
and then apply 3% human serum as a blocking agent (health hazard!). 4- incubate with the blocking for 10 min. 5- blot the slides without washing and apply Phycoerythrin (B-Pe or R-Pe) 0.5µg/ml in PBS-BSA-NaN 3 , in a moist chamber, at RT for 1-18 hr.
剂，重悬在流式染色缓冲液或者 PBS 里面）。如果需要长时间保存样本，建议在样本做完表面染色和固定后，保存在专门的保存缓冲液中。但即便是有专门的保存 buffer，对于用了串联染料（如 APC/Cy7、PE/Cy7 等）染色的样本，我们还是建议不要长时间保存，很难避免补偿的「漂移（shift）」。左图：human PBMC 刺激完当天染色后上机检测；右图：同一样本，表染和固定后，保存在 Cyto-Last™ buffer 里面，14 天后做胞内染色并上机。Ki- 67 染色时，选择哪种固定方式？流式
Intracellular Immunofluorescent Staining for Flow Cytometry
ConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d) 2d/3d anti-CD3 (10ug/ml immobilized) + anti-CD28 (2ug/ml soluble) 5hr Brefeldin A JES5-16E3 , JES5-2A5 IL-12/IL-23 (p40)
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