H3570- DAPI and Hoechst Nuclei

DAPI is a fluorescent dye commonly used in molecular biology and cell biology research. DAPI is known for its ability to bind to DNA, specifically to the minor groove of double-stranded DNA. This dye emits blue fluorescence when excited by ultraviolet light, allowing researchers to visualize and stain DNA in various biological samples.

Hoechst dyes are DNA-specific fluorescent stains that bind to the minor groove of double-stranded DNA. They emit blue fluorescence when excited by ultraviolet light. These dyes are widely used for staining DNA in various biological samples.

The FluoroPure™-grade of Hoechst and DAPI powders are manufactured using stringent purification processes to minimize impurities that could interfere with fluorescence imaging. This ensures that the DAPI and Hoechst provides reliable and consistent results with minimal background noise. The >98% purity of FluoroPure grade reagents make them particularly suitable for demanding applications such as super-resolution microscopy and live-cell imaging, where precise and accurate visualization of DNA is essential.

DAPI (4',6-Diamidino-2-Phenylindole):

DAPI 4',6-diamidino-2-phenylindole is a blue fluorescent probe that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA, where its fluorescence is approximately 20-fold greater than in the non-bound state. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm and efficient contrasting in IHC staining and spatial transcriptomics and proteomics tissue imaging. DAPI is a classic nuclear counterstain for immunofluorescence microscopy, as well as an important component of high-content screening methods requiring cell-based quantitation of DNA content and segmentation (ex/em 341/452 nm).

DAPI as a dilactate powder is more water soluble than the dihydrochloride salt, and therefore a better choice for preparing stock solutions in water.

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