尿嘧啶DNA糖苷酶（UNG酶/UDG酶）1U/µl

目的基因片段的PCR扩增与克隆

结合的引物长度足够的条件下，其5’端碱基可不与模板DNA互补而呈游离状态，因此可在引物5’端加上限制性内切酶位点、启动子序列或其它序列等以便于PCR产物的分析克隆等，引物的5’端最多可加10个碱基而对PCR反应无影响。 PCR反应系统总体积10 μl，模板DNA的量为植物总DNA 10 ng。 PCR反应体系(10 μl) 各对引物的复性温度不一样，用x代替。延伸时间用y代替，其中片断长度如果大于1kb，y为1.5 min；大于2kb，y为2.0 min；片断长度如果小于1kb，y

Plant DNA methylation analysis by bisulfite genomic sequencing

(12000 X g); Discard supernatant, vacuum dry for 10 minutes at room temperature; Dissolve cut DNA in 10µl bidist water (0.25 or 2.5 µg/µl, respectively), store at -20°C; Bisulfite treatment The bisulfite solution

Whole genome amplification protocol for ChIP-chip

of reverse crosslinked, QIAquick purified DNA is lyophilized and resuspended in 10µl of H2 0 (comment 1 and comment 2); Add 2µl 1X Library Preparation Buffer to 10µl of input material; Transfer samples to strip tubes or individual thin walled