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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
南京赛泓瑞生物
- 检测方法：
ELISA
- 适应物种：
人
- 样本：
细胞培养上清、血清、血浆、尿液、组织样本等
- 规格：
48T/96T
产品名称：Human TNF related activation induced cytokine,TRANCE ELISA Kit
用途
该试剂盒用于体外定量检测人血清、血浆或其他相关生物液体中天然及部分Human TNF related activation induced cytokine,TRANCE ELISA Kit需自备的设备及试剂
1、450±10nm 滤光片的酶标仪(建议仪器使用前提前预热)
2、单道或多道微量加液器及吸头
3、稀释样品的EP 管
4、蒸馏水或去离子水
5、吸水纸
6、盛放洗液的容器
Human TNF related activation induced cytokine,TRANCE ELISA Kit
Human TNF related activation induced cytokine,TRANCE ELISA Kit标本的采集与保存:
Human TNF related activation induced cytokine,TRANCE ELISA Kit
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文献和实验, the analysis of transcription factor activation by either electrophoretic mobilityshift assay (EMSA) or reporter assay, and the analysis of TNF-induced genes by chromatin IP (ChIP).
Intracellular Cytokine Staining Protocol
stimulation period for induction of a given cytokine is variable and has to be determined. For example, the best time for detection of IL-6-producing cells by human LPS-activated monocytes is 6 hours, whereas IL-10 needs at least 24 hours stimulation
Production and Characterization of Receptor-Specific TNF Muteins
receptors (TNFR) (radioligand competition binding and Biacore). The biological activity of wild-type TNF and/or TNF muteins can be assessed in cellular assays. TNF-induced cytotoxicity toward murine L929s cells and human Kym39A6 cells is mediated
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