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文献和实验Molecular Analysis of Mutations in the Human HPRT Gene
The HPRT assay uses incorporation of toxic nucleotide analogs to select for cells lacking the purine scavenger enzyme hypoxanthine-guanine phosporibosyltransferase. A major advantage of this assay is the ability to isolate mutant cells
Gene Knockout Techniques(1) The Knockout Process
for mutations in unc-22Day 8 (Wednesday) unc-22 is a large gene that makes a good test target for mutagenesis. Mutations that knock out unc-22 produce a dominant "twitching" phenotype when the animals are exposed to 1% nicotine. The presence of twitchers
Mammalian Cell HPRT Gene Mutation Assay: Test Methods
of gene expression/function produces a HPRT − mutant. Human cells are readily used, and mechanistic studies using the HPRT test methodology with modifications, such as knock-out cell lines for DNA repair, can provide details of the mode of action (MOA
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