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文献和实验Molecular Analysis of Mutations in the Human HPRT Gene
The HPRT assay uses incorporation of toxic nucleotide analogs to select for cells lacking the purine scavenger enzyme hypoxanthine-guanine phosporibosyltransferase. A major advantage of this assay is the ability to isolate mutant cells
Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytes
is required for the formation of functional T-cell receptor genes in T cells and antibody genes in B cells. This is an inherently dangerous process, however, because double-strand breaks are introduced into the chromosomes. Molecular evidence indicates
Somatic Mutations and Aging: Methods for Molecular Analysis of HPRT Mutations
susceptibility factors seem to contribute to the complexity of mutational spectra. The X-linked HPRT (hypoxanthine phosphoribosyl transferase ) gene in human T lymphocytes has been considered as a suitable target for studying somatic in vivo mutations
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