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爱必信（上海）生物科技有限公司
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Cloning and Expression of Single-Chain Fragments (scFv) from Mouse and Rat Hybridomas
, including rat hybridomas (6 –8 ). However, some of the restriction sites (in particular Pst I and Barn HI) introduced for subsequent cloning were found to be frequently present as internal sites in the DNA coding for mouse-antibody-variable domains
Immunofluorescent Staining of Mouse and Rat Leukocytes
-up, please see description in "Procedure for Setting Compensation for Multi-Color Flow Cytometric Analysis". For reducing FcgII/IIIR-mediated antibody binding (or binding of SAv-PE or SAv-Cy-Chrome) which could contribute to background, the use of anti-mouse CD32/CD
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