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文献和实验Hybrid Resistance by Mouse NK Cells In Vitro
, leads to generation of lytic NK1.1+ Ly49- cells whose function is similar to that of fetal NK cells, i.e., they can lyse YAC-1 tumor cells and class I deficient cells, but cannot lyse normal lymphoblasts and lack Ly49 receptors (5 ). These two types
Studying Wound Repair in the Mouse
2 , or protocol 3 Normal goat serum (NGS; Vector Laboratories, cat. no. S1000) Anti‐cytokeratin 6 antibody (Abcam, cat. no. ab24646
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
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