PE-IF594 Armenian Hamster anti

AbC Anti-Rat/Hamster Bead Kit

1 μL   5)      Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6)     

T-cell dependent antigen specific in situ staining (Pe)

6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block

secondary antibody review -- data from 99 publications

Signaling 18   IgG   Alexa Fluor 488 immunocytochemistry 1:2000     18   IgG   Alexa Fluor 568 immunocytochemistry 1:2000     19 donkey IgG   HRP western blot 1:3000 detect antibody binding in mouse epithelial cells Amersham Biosciences 19 goat