PE Armenian Hamster anti-Mouse

secondary antibody review -- data from 99 publications

Signaling 18   IgG   Alexa Fluor 488 immunocytochemistry 1:2000     18   IgG   Alexa Fluor 568 immunocytochemistry 1:2000     19 donkey IgG   HRP western blot 1:3000 detect antibody binding in mouse epithelial cells Amersham Biosciences 19 goat

immunofluorescence staining

to ensure your second step reagent will recognize the primary antibody. For instance, if your primary antibody is mouse IgG2a andyour second step reagent is anti-mouse IgG1, then it will not work. Also some primary antibodies are made in hamster or rat