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- 英文名：
2',2-Difucosyllactose with DBCO (Linker-DBCO A)
- 库存：
100+
- 供应商：
上海维亦特生物科技有限公司
分子量:1128.18
性状：White powder
储存：室温
溶解性：in water, PBS or HEPES
来源：Synthesis
附件：MS
糖链结构：Fucα1-2Galβ1-4(Fucα1-2)Glc-O-(CH2)3-NH-DBCO
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
Protease inhibitor cocktail for ChIP (1000X) Proteinase K (20 mg/mL) (Roche Diagnostics) Reagents for agarose gel electrophoresis RIPA buffer for ChIP RNase A SDS (sodium dodecyl sulfate) (10%) T cell isolation buffer
PCR amplification of variable regions (SOE-Protocol)
wherby the amplified VH and VL are connected by a primer encoded (Gly4Ser)3-linker. For the amplification of the variable regions, three different murine primer sets can be used: VH front (MuPD 3, 4, 5, 6, 7. 8. 9. 10, 11 and 12, mix) and VH back (MuPD
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
of methods used to uncover insertion sites have previouslybeen described, including genomic DNA library screening(15), ligation-mediated PCR (6), inverse PCR (16), VISAtechnique (17), T-linker PCR (18), and single nucleotidepolymorphism-based mapping (19
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