7,9-Di-tert-butyl-1-oxaspiro[4

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

in a yield of 40% after P (III) oxidation and purification. Complete removal of the nucleobases, phosphates, and 2??O ?tert ?butyldimethylsilyl protecting groups leads to the desired propargylated c?di?GMP diester. Cycloaddition of a biotinylated azide

Synthesis of Triazole‐Nucleoside Phosphoramidites and Their Use in Solid‐Phase Oligonucleotide Synthesis

2‐Bromoethanol (12 ; Sigma‐Aldrich, cat. no. B65586) (2‐bromoethoxy)(tert ‐butyl)dimethylsilane, 99% pure (13 ; Sigma‐Aldrich, cat. no. 428426)

（共享）免疫组织化学染色FAQ

, perhaps still available from Baxter, contains pyrrolid-2-one, a polyol, a urea and a zinc salt; mode of action unknown. Mirsky's Fixative from National Diagnostics is an aqueous solution of a complex di-aldehyde (possible di-aldehyde starch); mode of action