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- CAS号：
675198-19-3
(4-(3-Methoxypropoxy)-3-methylpyridin-2-yl)methanol Hydrochloride
675198-19-3
C₁₁H₁₇NO₃ • HCl
N/A
Off-White to Pale Yellow Solid
Chloroform (Slightly), Methanol (Slightly)
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文献和实验(4-(3-Methoxypropoxy)-3-methylpyridin-2-yl)methanol Hydrochloride
675198-19-3
C₁₁H₁₇NO₃ • HCl
N/A
Off-White to Pale Yellow Solid
Chloroform (Slightly), Methanol (Slightly)
Guanidine Hydrochloride Purification of Proteins From SDS
Pepstatin A (2.0 mg/ml)10 ml Leupeptin (1.0 mg/ml)10 ml Aprotinin (5.0 mg/ml)(Protease Inhibitor Cocktail Sigma #P8340)Denaturation Buffer6 M guanidine hydrochloride 57.3 g G-HCl20 mM Hepes 7.9 2 ml 1M Hepes pH 7.910 mM KCl 0.074 g KCl0.5 mM DTT 100 ml 0.5 M
Guanidine Hydrochloride Purification of Proteins From SDS PAGE
(Sigma #P7626) 10 ml Pepstatin A (2.0 mg/ml) 10 ml Leupeptin (1.0 mg/ml) 10 ml Aprotinin (5.0 mg/ml) (Protease Inhibitor Cocktail Sigma #P8340) Denaturation Buffer 6 M guanidine hydrochloride 57.3 g G-HCl 20 mM Hepes 7.9 2 ml 1M Hepes pH 7.9 10 mM
serve as extraction standards and are added to the blanks, controls and patient samples prior to protein precipitation with methanol. The extracts are evaporated to complete dryness and derivatized in two steps using methoximation with methoxylamine
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