相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
DNA-Free Taq 2×SuperMix contains DNA-Free Taq DNA polymerase, dNTPs, and an optimized reaction buffer at a 2× concentration. For DNA amplification, simply add the template, primers, and water to make the 2× SuperMix solution a 1× concentration to proceed with the reaction. The amplification products have an "A" base at the 3' end, making them directly suitable for gene cloning. DNA-Free Taq 2× SuperMix (dye) contains electrophoresis dye, allowing amplification products to be directly loaded for electrophoresis. If used for cloning, the dye needs to be purified away.
Cat. No.: FTM-5 (for 5ml)
Cat. No.: FTM-20 (for 20ml)
Cat. No.: FTM-50 (for 50ml)
Description
DNA-Free Taq 2×SuperMix contains DNA-Free Taq DNA polymerase, dNTPs, and an optimized reaction buffer at a 2× concentration. For DNA amplification, simply add the template, primers, and water to make the 2× SuperMix solution a 1× concentration to proceed with the reaction. The amplification products have an "A" base at the 3' end, making them directly suitable for gene cloning. DNA-Free Taq 2× SuperMix (dye) contains electrophoresis dye, allowing amplification products to be directly loaded for electrophoresis. If used for cloning, the dye needs to be purified away.
Features
- Completely free from bacterial DNA.
- Reduces PCR amplification time.
- Prevents contamination from multiple steps.
- Suitable for amplifying genomic DNA fragments (≤4 kb).
Applications
- Routine PCR amplification.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验。 a.时间越长,DNA的产量越多,污染也越多 7. 12000转速离心5-10min,去上清,倒置于吸水纸上数分钟,加入700µl 70%的酒精,上下颠倒数次,洗涤沉淀。12000转离心5-10m,去上清,倒置于吸水纸上数分钟。重复一遍。 8. 12000转速离心5-10min,去上清,倒置于吸水纸上数分钟,最后置于超净工作台中吹干。 9. 在离心管中加入30-50µl的灭菌去离子水或者TE溶液,4℃放置数小时,使其充分溶解。 10
for both DNA sample and reaction mixture preparation, is strongly recommended.The reagents for PCR should be prepared separately and used solely for this purpose. Autoclaving of all solutions, except dNTPs, primers and Taq DNA Polymerase is recommended
DNA聚合酶Taq Plus和 Taq Plus I 有什么区别吗?对PCR结果有影响吗? Taq Plus 集扩增效率高和保真度好于一体。能有效地扩增10 kb以下的片段,扩增效率比Pfu高,保真度 Taq Plus 比Taq好。对于有些结构复杂的模板,如GC含量高、有二级结构等可选用Taq Plus扩增。 Taq
技术资料暂无技术资料 索取技术资料










