DNA-Free Thermo-stable M-MLV R

Thermo-stable M-MLV Reverse Transcriptase (RNase H-) is an upgraded version of Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase. It possesses RNA-dependent DNA polymerase activity, DNA-dependent DNA polymerase activity, and lacks RNase H activity. The RNA-dependent DNA polymerase activity allows the synthesis of complementary DNA (cDNA) from an RNA template, while the DNA-dependent DNA polymerase activity enables the synthesis of double-stranded DNA from a cDNA single strand. The thermal stability of Thermo-stable M-MLV Reverse Transcriptase (RNase H-) is significantly enhanced, retaining over 50% of its enzymatic activity at 55-60°C. This enzyme has a stronger extension capability, making it suitable for synthesizing longer cDNA. In contrast,  conventional MMLV Reverse Transcriptase retains only 40-50% activity at 45°C and just 10% at 50°C.

Cat. No.: DFMM-10k (for 10KU) 

Cat. No.: DFMM-50k (for 50KU) 

Cat. No.: DFMM-200k (for 200KU) 

Cat. No.: DFMM-500k (for 500KU) 

The glycerol-free version is also available for this product.

For M-MLV Reverse Transcriptase active at 65°C, please see Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-).

For M-MLV Reverse Transcriptase active at 72°C, please see Prime Thermo-stable M-MLV Reverse Transcriptase (RNase H-).

Description

Thermo-stable M-MLV Reverse Transcriptase (RNase H-) is an upgraded version of Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase. It possesses RNA-dependent DNA polymerase activity, DNA-dependent DNA polymerase activity, and lacks RNase H activity. The RNA-dependent DNA polymerase activity allows the synthesis of complementary DNA (cDNA) from an RNA template, while the DNA-dependent DNA polymerase activity enables the synthesis of double-stranded DNA from a cDNA single strand. The thermal stability of Thermo-stable M-MLV Reverse Transcriptase (RNase H-) is significantly enhanced, retaining over 50% of its enzymatic activity at 55-60°C. This enzyme has a stronger extension capability, making it suitable for synthesizing longer cDNA. In contrast,  conventional MMLV Reverse Transcriptase retains only 40-50% activity at 45°C and just 10% at 50°C.

Our DNA-Free Thermo-stable M-MLV Reverse Transcriptase (RNase H-), on the other hand, is devoid of genomic and plasmid DNA from the production strain. This makes DNA-Free Thermo-stable M-MLV Reverse Transcriptase (RNase H-) particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes, as well as for standard applications like RT-PCR.

Activity Definition

One unit (U) of enzyme activity is defined as the amount of enzyme required to incorporate 1 nmol of [3H] dTTP into Poly(A) - Oligo(dT) template/primer in 10 minutes at 37°C.

Concentration

200U/μl

Features

• Lacks RNase H activity.
• Superior cDNA synthesis length compared to wild-type M-MLV reverse transcriptase.
• Excellent thermal stability: withstands reactions at 55-60°C.

Applications

• First-strand cDNA synthesis
• Full-length cDNA synthesis and cDNA library preparation
• RT-PCR and Real-Time RT-PCR reactions

Storage

Store at -20°C