DNA-Free Thermolabile Calf Int

DNA-Free Thermolabile Calf Intestinal Alkaline Phosphatase (CIP) is a heat-labile recombinant calf intestinal alkaline phosphatase (CIP) that catalyzes the dephosphorylation of 5' and 3' phosphate monoesters of DNA and RNA. Additionally, Rapid CIP hydrolyzes ribonucleoside and deoxyribonucleoside triphosphates (NTPs and dNTPs). Rapid CIP is widely used in molecular biology research for removing phosphate groups from DNA and RNA ends, facilitating subsequent cloning and probe end-labeling. In cloning, it dephosphorylates linear vectors to prevent self-ligation. This enzyme acts quickly on 5' overhangs, recessed ends, and blunt ends, with a reaction time of just 10 minutes. Rapid CIP can also degrade free dNTPs in PCR reactions, making it suitable for preparing sequencing templates and SNP analysis. Compared to wild-type CIP, Rapid CIP is 100% irreversibly inactivated by heating at 80°C for 2 minutes without the need for additional reagents such as magnesium ions or EDTA. Therefore, no purification is required before ligation or end-labeling.

Cat. No.: DFCIP-1k (for 1000U)

Cat. No.: DFCIP-5k (for 5000U)

Description

DNA-Free Thermolabile Calf Intestinal Alkaline Phosphatase (CIP) is a heat-labile recombinant calf intestinal alkaline phosphatase (CIP) that catalyzes the dephosphorylation of 5' and 3' phosphate monoesters of DNA and RNA. Additionally, Rapid CIP hydrolyzes ribonucleoside and deoxyribonucleoside triphosphates (NTPs and dNTPs). Rapid CIP is widely used in molecular biology research for removing phosphate groups from DNA and RNA ends, facilitating subsequent cloning and probe end-labeling. In cloning, it dephosphorylates linear vectors to prevent self-ligation. This enzyme acts quickly on 5' overhangs, recessed ends, and blunt ends, with a reaction time of just 10 minutes. Rapid CIP can also degrade free dNTPs in PCR reactions, making it suitable for preparing sequencing templates and SNP analysis. Compared to wild-type CIP, Rapid CIP is 100% irreversibly inactivated by heating at 80°C for 2 minutes without the need for additional reagents such as magnesium ions or EDTA. Therefore, no purification is required before ligation or end-labeling.

Our DNA-Free enzymes are all recombinant proteins and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.

Unit Definition

One unit is defined as the amount of enzyme required to hydrolyze 1 μmol of p-nitrophenyl phosphate  to p-nitrophenol in a 1 ml reaction system at 37℃ within 1 minute.

Features

• Completely free from bacterial DNA.
• Rapid and irreversible heat inactivation, saving purification steps.
• Superior stability compared to wild-type enzymes.
• No need to add zinc or other additives; reaction time is just 10 minutes.
• Flexible reaction conditions compatible with any restriction enzyme buffer, no purification needed.
• Higher activity requiring less enzyme, reducing reaction costs.
• Eliminates the need for multiple phosphatases; Rapid CIP can remove 5' and 3' phosphates from DNA, RNA, and dNTPs.
• Degrades unincorporated dNTPs in PCR products, improving DNA sequencing and SNP analysis quality.

Storage

Store at -20℃ for up to 2 years. Avoid repeated freeze-thaw cycles.