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- DFAPE-50 (for 50U)
- 2026年04月10日
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DNA-Free Alkaline Phosphatase (E. coli) catalyzes the hydrolysis of all phosphate monoesters but does not catalyze the hydrolysis of phosphate diesters or triesters. It also catalyzes the hydrolysis of pyrophosphate bonds in compounds such as ATP, primarily used for the removal of 5’ phosphate groups from DNA and RNA ends.
Cat. No.: DFAPE-50 (for 50U)
Cat. No.: DFAPE-250 (for 250U)
Description
DNA-Free Alkaline Phosphatase (E. coli) catalyzes the hydrolysis of all phosphate monoesters but does not catalyze the hydrolysis of phosphate diesters or triesters. It also catalyzes the hydrolysis of pyrophosphate bonds in compounds such as ATP, primarily used for the removal of 5’ phosphate groups from DNA and RNA ends.
Our DNA-Free enzymes are all recombinant proteins and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.
Unit Definition
One unit is defined as the amount of enzyme required to hydrolyze 1 μmol of p-nitrophenyl phosphate to p-nitrophenol within 1 minute at 25℃ in 1× Alkaline Phosphatase reaction buffer. The specific activity of this enzyme is approximately 100 U/mg.
Features
- Completely free from bacterial DNA.
- Removal of 5’ phosphate groups from RNA or DNA before 32P labeling.
- Dephosphorylation of vector DNA fragments to facilitate ligation with T4 DNA Ligase.
Storage
Store at -20℃ for up to 2 years. Avoid repeated freeze-thaw cycles.
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文献和实验Expression of Recombinant Alkaline Phosphatase Conjugates in Escherichia coli
The methods described in this article are relative to the use of a positive cloning/screening recombinant system for the generation in Escherichia coli of foreign proteins fused to a highly active bacterial alkaline phosphatase (PhoA) variant
Isolation of Plasmids from E. coli by Alkaline Lysis
Purification of plasmid DNA from Escherichia coli using alkaline lysis (1 ,2 ) is based on the differential denaturation of chromosomal and plasmid DNA in order to separate the two. Bacteria are lysed with a solution containing sodium
in solution c. for 5-15 minutes at room temperature. Wash, counterstain in haematoxylin, wash. Mount in phosphate buffered glycerine jelly RESULTS Alkaline phosphatase activity - Red Nuclei - Blue
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