- ￥1268
- abinScience
- France
- ZA444012
- 2026年03月03日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期：
1 year
- 英文名：
Recombinant Zebrafish caspase-8 Protein, N-His
- 库存：
999
- 供应商：
abinScience
- 规格：
50ug
| Product name | Recombinant Zebrafish caspase-8 Protein, N-His |
|---|---|
| Catalog No. | ZA444012 |
| Host species | Lyophilized |
| Specificity | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Conjugation | >90% as determined by SDS-PAGE. |
| Species reactivity | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Immunogen | Please contact with the lab for this information. |
| Form | E. coli |
| Storage buffer | AAS91705.1 |
| Purity | Met1-Gly186 |
| Clonality | Recombinant |
| Isotype | 23.88 kDa |
| Applications | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Target | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Purification | caspase-8, caspase-8 |
| Accession | Danio rerio (Zebrafish) |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验信息 1、Recombinant Gene Expression : Reviews and Protocols Editor(s): Paulina Balbás1, Argelia Lorence2 Affiliation(s): (1),Centro de Investigación en Biotecnología,CuernavacaMéxico (2)Virginia Polytechnic Institute,State University,BlacksburgVA
如下：几丁质结合蛋白域―intein1―MCS―Intein2―几丁质结合蛋白。这个巧妙的设计有3重便利：N-端，C-端或者N-端和C-端同时融合短的内含肽序列。想做N端融合，你可以将目的基因替换MCS―Intein2―几丁质结合蛋白，如果想做C端融合表达，就可以将目的基因替换MCS前面的几丁质结合蛋白域―intein1―MCS，不用分2个载体；而且做过克隆的人会很有体会：很多时候双酶切载体，由于2个酶切位点都多克隆位点上，没有小片断切下来，所以是否成功双酶切就不好判断，如果用Twin载体就会切下一个片断
内的死亡域（DD）结合Fas结合蛋白FADD（fas associated protein with DD），FADD再以其N端的死亡效应子域（death effector domain，DED）结合Pro-Caspase-8（或-10），形成一个由Fas-FADD-Pro-Caspase-8（或-10）三种分子组成的复合体，称为诱导死亡的信号复合体（death inducing signaling complex，DISC），其中Pro-Caspase-8（或-10）就可以自身催化
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