- ￥1268
- abinScience
- France
- ZA438012
- 2026年03月03日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期：
1 year
- 英文名：
Recombinant Zebrafish sirt1 Protein, N-His
- 库存：
999
- 供应商：
abinScience
- 规格：
50ug
| Product name | Recombinant Zebrafish sirt1 Protein, N-His |
|---|---|
| Catalog No. | ZA438012 |
| Host species | Lyophilized |
| Specificity | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Conjugation | >90% as determined by SDS-PAGE. |
| Species reactivity | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Immunogen | Please contact with the lab for this information. |
| Form | E. coli |
| Storage buffer | XP_001334440.4 |
| Purity | Asn177-Tyr438 |
| Clonality | Recombinant |
| Isotype | 32.16 kDa |
| Applications | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Target | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Purification | NAD-dependent protein deacetylase sirtuin-1, sirt1 |
| Accession | Danio rerio (Zebrafish) |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验信息 1、Recombinant Gene Expression : Reviews and Protocols Editor(s): Paulina Balbás1, Argelia Lorence2 Affiliation(s): (1),Centro de Investigación en Biotecnología,CuernavacaMéxico (2)Virginia Polytechnic Institute,State University,BlacksburgVA
如下：几丁质结合蛋白域―intein1―MCS―Intein2―几丁质结合蛋白。这个巧妙的设计有3重便利：N-端，C-端或者N-端和C-端同时融合短的内含肽序列。想做N端融合，你可以将目的基因替换MCS―Intein2―几丁质结合蛋白，如果想做C端融合表达，就可以将目的基因替换MCS前面的几丁质结合蛋白域―intein1―MCS，不用分2个载体；而且做过克隆的人会很有体会：很多时候双酶切载体，由于2个酶切位点都多克隆位点上，没有小片断切下来，所以是否成功双酶切就不好判断，如果用Twin载体就会切下一个片断
表观遗传修饰：想要改变基因表达？不只有 DNA，染色体也可以！
推荐 DNMT1蛋白, Human (HY-P703564) www.medchemexpress.cn/recombinant-proteins/dnmt1-protein-human.html 重组的 DNMT1 蛋白 P26358 (P351-A1600) 片段，由 E. coli 表达，带有 tag Free 标签。 DNMT2蛋白, Human (sf9, GST) (HY-P75711) www.medchemexpress.cn/recombinant-proteins/dnmt
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