- ￥1268
- abinScience
- France
- HB623022
- 2026年03月03日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期：
1 year
- 英文名：
Recombinant Human CCNB1 Protein, N-GST & C-His
- 库存：
999
- 供应商：
abinScience
- 规格：
50ug
| Product name | Recombinant Human CCNB1 Protein, N-GST & C-His |
|---|---|
| Catalog No. | HB623022 |
| Host species | Lyophilized |
| Specificity | Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Conjugation | >90% as determined by SDS-PAGE. |
| Species reactivity | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Immunogen | Please contact with the lab for this information. |
| Form | E. coli |
| Storage buffer | P14635 |
| Purity | Val171-Val433 |
| Clonality | Recombinant |
| Isotype | 58.44 kDa |
| Applications | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Target | Reconstitute in sterile water for a stock solution. |
| Purification | CCNB1, G2/mitotic-specific cyclin-B1, CCNB |
| Accession | Homo sapiens (Human) |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验Small Scale GST-Tag Purification Under Nature Conditions
Small Scale GST-Tag Purification Under Nature Conditions According to AMERSHAM-BIOSCIENCES-GST fusion protein Handbook (pdf) Aliquot of Cell Pellet after Induction The idea is to aliquot cells after induction
-S10 柱子对于去污剂有高度的结合容量，比如每毫升固定的树脂可以结合 6 mg SDS 和 14 mg Triton® X-100。Table 1: A comparison of the detergent removal rates and percentage protein recovery with DetergentOUT™ GBS10. ag 蛋白的纯化 带有融合标签的表达产物----融合蛋白的纯化通常也是紧接细胞破碎工作进行的。设计融合标签的主要目的之一就是为了方便
[资源][原创] 蛋白标签纯化技术——CBP（钙调蛋白结合肽）
缓冲液中能够特异性的被钙调蛋白树脂捕捉吸附，并且在中性环境中能够被2 mM EGTA洗脱，这与6组氨酸亲合标签纯化体系相比反应条件要温和许多。仅有4-kDa 大小的CBP tag 与26-kDa GST 标签相比对蛋白分子的影响非常小。在所表达的蛋白分子上包含一个凝血酶或肠激酶列解位点，依靠它可以非常方便的移除CBP标签。在哺乳动物细胞中表达的标签纯化系统(两步纯化法用于高纯度的要求)Mammalian TAP System本系统同时用到了streptavidin binding peptide
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