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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温运输,-20℃保存,12个月有效。
- 保质期:
低温运输,-20℃保存,12个月有效。
- 英文名:
核酸助沉剂(Glycogen,5mg/ml)
- 库存:
999
- 供应商:
麦格生物
- 规格:
1ml
注意事项:5ul Glycogen (5mg/ml)即可把少至皮克(pg)级的DNA或RNA从1ml的溶液体系中沉淀出来。用glycogen沉淀后对于后续的细菌转化没有干扰,0.001mg/ml glycogen不会抑制TdT
储运及效期:低温运输,-20℃保存,12个月有效。
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文献和实验Clonality - X Chromosome Inactivation Assay
(1:1:1, v:v:v) (Gibco) 3 M Na Acetate, pH 5.2 (Life Technologies) Isopropanol Glycogen, 10 mg/ml (GenHunter) 70% ethanol Buffer 1 (50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 50 mM NaCl) Hha I restriction enzyme (Life Technologies) REact 2 Buffer
mL of primary antibodydilution buffer containing one of the following antibodies andincubate as indicated: phospho-b-catenin (dilution 1:1,000;overnight), phospho-glycogen synthase (dilution 1:1,000; 1 hat RT), GSK-3a (dilution 1:1,000; 1 h at RT
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
. If duplicates are used, transfer culture fluid from the duplicate block to the first block. Spin again, decant and drain. 3. Resuspend each pellet in 300ul of ice cold Qiagen buffer R1 with RNAse A (0.2 mg/ml) and RNAse T1 (100 units/ml). Let rest for 10
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