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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Long trebler phosphoramidite, 250 mg
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
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- 规格：
250mg
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文献和实验to precipitate as long as desired （up to overnight）。 To proceed, spin sample in a 4 !C microfuge at max speed for 30 minutes to 1 hour. 12. Aspirate off supernatant and wash pellet with 250 L 70% EtOH and spin down 5 . polyA RNA Isolation 13. Aspirate
PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
, and may also result in inadvertent “asymmetric” or single-strand amplification of the most efficiently primed product strand.Annealing does not take long: most primers will anneal efficiently in 30 sec or less, unless the Ta is too close to the Tm
while retaining the oligo-dT bound poly（A）-RNA. After several washing steps under conditions less stringent than for annealing, poly（A）-RNA is eluted in water or low salt Tris buffer. Elution volumes vary from 20-250 μl for most kits accepting up to 1 mg of total
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