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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Long trebler phosphoramidite, 500 mg
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
500mg
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文献和实验Long PCR Reagents and Guidelines
) and 5% DMSO (1% final concentration) can easily be made using 500 mM Mg(OAc)2, 1 M Tricine and 1 M KOAc stock solutions as follows: 5X Long PCR Buffer 425 mM KOAc 125 mM Tricine pH 8.7 (adjust pH of Tricine stock solution with KOH) 40% glycerol
Long PCR Reagents and Guidelines
final concentration) and 5% DMSO (1% final concentration) can easily be made using 500 mM Mg(OAc)2, 1 M Tricine and 1 M KOAc stock solutions as follows: 5X Long PCR Buffer 425 mM KOAc 125 mM Tricine pH 8.7 (adjust pH of Tricine
High Efficiency Yeast Transformation (Gietz Long Protocol)
the LiAc with a micropipette.8. Resuspend the cells to a final volume of 500 µl (2 x 109 cells/ml) --about 400µl of 100 mM LiAc--Note: If the cell titer of the culture is greater than 2 x 107/ cells ml the volume of the LiAc should be increased to maintain
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