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- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Biotin-11-UTP, 250 nmol
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
250nmol
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文献和实验Making RNA probes for in situ hybridization
2 μl 10 mM GTP 100mM 2 μl 10 mM UTP 100mM 1.3 μl 6.5 mM DNP-11-UTP 10mM 7.0 μl 3.5 mM In vitro
in the nucleus in human cell cultures we designed several RNA-biotin based pulldown assays which can be used alone or in combination with other known assays such as ChIP and Flag-tagged pulldown assays. Three protocols are explained
The ribonuclease protection assay (RPA)
, the probe synthesis is performed during the afternoon Day 1, hybridizations are incubated overnight, and RNase treatments and gel electrophoresis are performed early on Day 2. Probe Synthesis: 1. Bring the [a-32P]UTP, GACU nucleotide pool, DTT
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