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- BHcell（博辉生物）
- BH-C477
- 2026年01月16日
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T25
AtT-20/AtT-20细胞系/AtT-20细胞株/AtT-20小鼠垂体瘤细胞
Cell line name AtT-20
Synonyms AtT 20; ATT-20; AtT20
Accession CVCL_2300
Resource Identification Initiative To cite this cell line use: AtT-20 (RRID:CVCL_2300)
Comments Doubling time: ~3 days (Note=Lot 11152004) (JCRB=JCRB9098).
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Derived from site: In situ; Pituitary gland; UBERON=UBERON_0000007.
Disease Mouse pituitary gland neoplasms (NCIt: C24037)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: LAF1.
Hierarchy Children:
CVCL_XA98 (AtT-20-FlpIn) CVCL_4W08 (AtT-20/D16v) CVCL_C6XD (AtT-20/HNP)
CVCL_1G40 (AtT-20ins) CVCL_1G39 (AtT-20ins4b/1)
Category Cancer cell line
STR profile Source(s): Procell
Markers:
Mouse STR 1-1 11,15
Mouse STR 1-2 13,17
Mouse STR 2-1 15,16
Mouse STR 3-2 10,14
Mouse STR 4-2 18.3
Mouse STR 5-5 16,18
Mouse STR 6-4 18
Mouse STR 6-7 13,15
Mouse STR 7-1 25.2
Mouse STR 8-1 15
Mouse STR 11-2 15,16
Mouse STR 12-1 16,17
Mouse STR 13-1 17.2,18.2
Mouse STR 15-3 17,22.3
Mouse STR 17-2 14
Mouse STR 18-3 17,18
Mouse STR 19-2 11,12
Mouse STR X-1 25
Run an STR similarity search on this cell line
Publications
PubMed=13874682; DOI=10.1073/pnas.48.7.1184; PMCID=PMC220930
Buonassisi V., Sato G.H., Cohen A.I.
Hormone-producing cultures of adrenal and pituitary tumor origin.
Proc. Natl. Acad. Sci. U.S.A. 48:1184-1190(1962)
DOI=10.1007/BF02618370
Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)
PubMed=6317196; DOI=10.1016/0092-8674(83)90187-3
Moore H.-P.H., Walker M.D., Lee F., Kelly R.B.
Expressing a human proinsulin cDNA in a mouse ACTH-secreting cell Intracellular storage, proteolytic processing, and secretion on stimulation.
Cell 35:531-538(1983)
PubMed=2177466; DOI=10.1007/BF02624442
Vedder H.
Serum-free culture of AtT 20 pituitary cells: a system for neuroendocrine studies under defined conditions.
In Vitro Cell. Dev. Biol. 26:1068-1072(1990)
PubMed=8383976; DOI=10.1677/jme.0.0100025
Farrell W.E., Stewart M.F., Clark A.J.L., Crosby S.R., Davis J.R.E., White A.
Glucocorticoid inhibition of ACTH peptides: small cell lung cancer cell lines are more resistant than pituitary corticotroph adenoma cells.
J. Mol. Endocrinol. 10:25-32(1993)
PubMed=15541569; DOI=10.1016/j.mce.2004.07.018
Ooi G.T., Tawadros N., Escalona R.M.
Pituitary cell lines and their endocrine applications.
Mol. Cell. Endocrinol. 228:1-21(2004)
PubMed=21214938; DOI=10.1186/1471-2164-12-14; PMCID=PMC3024950
Atwood B.K., Lopez J., Wager-Miller J., Mackie K., Straiker A.
Expression of G protein-coupled receptors and related proteins in HEK293, AtT20, BV2, and N18 cell lines as revealed by microarray analysis.
BMC Genomics 12:14.1-14.14(2011)
PubMed=25277546; DOI=10.1186/1471-2164-15-847; PMCID=PMC4198738
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. 3rd, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)
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文献和实验*发表【中文论文】请标注：由博辉生物科技（广州）有限公司提供； *发表【英文论文】请标注：From Bohui Biological Technology (Guangzhou) Co., Ltd.
白细胞介素 IL-20 IL-20主要产生细胞：角质形成细胞 IL-20氨基酸数目：178 IL-20分子质量(kDa)：35．2 IL-20受体：IL-22Rα；IL-20Rβ IL-20主要生物学活性：属IL10家族细胞因于，是角质形成细胞的自分泌因子，调节角质形成细胞在炎症反应中的作用。其受体由两个亚基组成，均为n型细胞因子受体超家族成员。IL20R可结合II--19
Genotyping using Affymetrix arrays
BioLabs) in volumes of 20 µL for 2 hours at 37 °C. Following heat inactivation at 70 °C for 20 minutes, 0.25 µM of XbaI adaptor (5’-ATT ATG AGC ACG ACA GAC GCC TGA TCT-3’ and 5’phosphate CTA GAG ATC AGG CGT CTG TCG TGC TCA TAA-3’)(Affymetrix), or HindIII
Geno typing using Affymetrix arrays
, 0.25 µM of XbaI adaptor (5’-ATT ATG AGC ACG ACA GAC GCC TGA TCT-3’ and 5’phosphate ?CTA GAG ATC AGG CGT CTG TCG TGC TCA TAA-3’)(Affymetrix), or HindIII adaptor (5’-ATT ATG AGC ACG ACA GAC GCC TGA TCT-3’ and 5’phosphate ?AGC TAG ATC AGG CGT CTG TCG TGC
技术资料